Efficacy comparison of peracetic acid 2%and sodium hypochlorite 5.25% in the smear layer removal of root canal instrumented with three rotary systems

• Grant number: 12/05787-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2012
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Ana Paula Martins Gomes
• Grantee: Caterina Ré da Silva
• Host Institution: Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The objective of this study will be evaluated, by scanning electron microscopy analysis, the effect of peracetic acid solution 2% and sodium hypochlorite solution 5.25% in the root canal walls after rotary instrumentation. Will be used 60 single-rooted human roots with standardized length and will have their canals instrumented by three rotary systems (Biorace, MTwo and Endowave), varying the irrigation solution, as follows (n=10): G1- Instrumentation with Biorace System + sodium hypochlorite solution 5.25%; G2- Instrumentation with MTwo System + sodium hypochlorite solution 5.25%; G3- Instrumentation with Endowave System + sodium hypochlorite solution 5.25%; G4- Instrumentation with Biorace System + peracetic acid solution 2%; G5- Instrumentation with MTwo System + peracetic acid solution 2%; G6 - Instrumentation with Endowave System + peracetic acid solution 2%. After instrumentation, the roots will be cleaved and the dentin walls will be evaluated by SEM with 500x and 2000x magnification for evaluation the cleaning of the root canals at the cervical, middle and apical thirds. The analyzed area will be quantified according by the total number of tubules present and the percentage of open tubules at each region. Data will be submitted to statistical analysis by ANOVA and Tukey tests, at the significance level of 5%.