Study of structure, immunoreactivity and protein partners of proteins encoded by micro-exon genes of Schistosoma mansoni

• Grant number: 12/07288-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2012
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Ricardo de Marco
• Grantee: Débora Orcia
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 08/03181-8 - Study of genes involved in the host-parasite relationship in Schistosoma mansoni, AP.JP

Abstract

In this DD project we will study and characterize some groups of proteins secreted by S. mansoni worm, the responsible for schistosomiasis, a disease that afflicts the humanity for centuries. It is believed that proteins secreted by the parasite are "key parts" to the action of a mechanism of modulation of the immune response of the host, which enables that the parasite can persist for decades within the host without the manifestation of an infectious process. These proteins, formed by micro-exon genes, were found in secretions of schistosomulum and the parasite egg, showing that these are exposed to the immune system of man. Understanding this possible modulation may enable the creation of a new front against to the parasite and enables a better understanding of the immune system of man. The project will be based on the expression in heterologous systems of protein products of micro-exons genes and the characterization of these structures through specific techniques such as circular dichroism, dynamic light scattering and crystallization trials. We will perform immunological tests, such as "dot-Blotting" and "ELISA", that will used to detect possible antibodies that act against the expressed recombinant protein, from serum of infected animals. Tests for MEG14 interactions with proteins S100A8/A9 will also run, and the interactions of PCMs with phospholipid vesicles will be verified. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, p. 8-pg., 2017-01-01. (12/07288-7, 13/20715-4, 14/09361-9, 12/09186-7, 10/20290-5)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, A, p. 3490-3497, JAN 2017. (12/07288-7, 13/20715-4, 14/09361-9, 10/20290-5, 12/09186-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ORCIA, Débora. Study of structure and protein partners of proteins coded by micro-exon genes of Schistosoma mansoni. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.