Advanced search
Start date

Identification through phage display with cDNAs synthetic library of antigenic epitopes of Schistosoma mansoni targeted by the immune response of Rhesus monkeys (Macaca mulatta) infected by the parasite and self-cured

Grant number: 19/09404-3
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2019
Effective date (End): April 30, 2023
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Sergio Verjovski Almeida
Grantee:Daisy Woellner Santos
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Schistosomiasis is currently considered the second leading Neglected Tropical Disease (NTD), and is present in 78 countries. It is estimated that around 54 million people are infected with the species Schistosoma mansoni and 393 million living in an area of risk, with praziquantel being the only drug available for the treatment of this parasite. The present research project aims to identify parasite proteins that are the targets of the immune response of S. mansoni infected Rhesus monkeys, since in these animals the course of infection starts normally, and after a few weeks the monkeys are able to self-heal and develop a strong resistance to a second challenge. We will use a synthetic library of oligos (cDNAs) that encodes all proteins known of the parasite. For the oligos library, fragments of all ORFs will be synthesized by releasable DNA microarray technique, totaling approximately 120,000 sequences covering 13,000 genes encoding parasite proteins. Protein fragments encoded by these oligos will be expressed as fusion proteins in the bacteriophage PG8.2 viral capsid by the phage display technique and unbiased screening of antigenic epitopes will be performed by exposing the phage library to the antibodies present in the collected plasma along the infection and reinfection (challenge) of each of 12 infected and self-cured Rhesus monkeys. Phage bound to the plasma antibodies will be immunoprecipitated and their cDNA inserts will be sequenced by the PhIP-Seq technique. The results of the sequencing will be analyzed by bioinformatics tools and correlated with the process of self-healing and resistance to challenge, with the intention of discovering new vaccine candidate targets for Schistosomiasis. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AMARAL, MURILO SENA; SANTOS, DAISY WOELLNER; PEREIRA, ADRIANA S. A.; TAHIRA, ANA CAROLINA; MALVEZZI, JOAO V. M.; MIYASATO, PATRICIA AOKI; FREITAS, RAFAELA DE PAULA; KALIL, JORGE; FAT, ELISA M. TJON KON; DE DOOD, CLAUDIA J.; et al. Rhesus macaques self-curing from a schistosome infection can display complete immunity to challenge. NATURE COMMUNICATIONS, v. 12, n. 1, . (16/10046-6, 15/06366-2, 19/09404-3, 18/15049-9, 18/18117-5, 18/23693-5)

Please report errors in scientific publications list using this form.