Generation of induced pluripotent cells of patients with autistic disorder

• Grant number: 11/20683-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2012
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Patricia Cristina Baleeiro Beltrão Braga
• Grantee: Fabiele Baldino Russo
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 13/08844-3 - Morphological and functional research of neurons derived from induced pluripotent stem cells from autistic spectrum disorder patients, BE.EP.DR

Abstract

The elucidation of genetic disease whose cause is not fully established is mainly hampered by poor or lack of adequate models for such studies. In 2006 a group of researchers, using an ingenious protocol, managed to reprogram differentiated adult cells, turning them into cells with characteristics similar to embryonic cells using genes that are expressed in pluripotent stem cells by transduction with retroviral vectors, and called these cells as induced pluripotent stem cells (iPS). Later, in 2009, using that technology, other groups showed that they could differentiate iPS cells in specific cellular types, cellular targets, creating thus models for in vitro study of diseases. In this work we aim to induce reprogramming of human epithelial cells from oral mucosa from patients with and without autism spectrum disorder (ASD), a disease still not fully understood by genetic field which shows high incidence. Currently, it is estimated that 1% of all school-age children have ASD. It is a disorder that affects the psycho-neurological development and affects a person's ability to communicate, understand and speak thus affecting their social life. In addition, our goal in this project is to reprogram cells from patients with ASD, characterize these cells, differentiate them into neurons and then compare the cellular profile, creating an in vitro model for studying ASD.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSA-FERNANDES, LIVIA; CUGOLA, FERNANDA RODRIGUES; RUSSO, FABIELE BALDINO; KAWAHARA, REBECA; DE MELO FREIRE, CAIO CESAR; CORREA LEITE, PAULO EMLIO; BASSI STERN, ANA CAROLINA; ANGELI, CLAUDIA BLANES; LEAL DE OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; et al. Zika Virus Impairs Neurogenesis and Synaptogenesis Pathways in Human Neural Stem Cells and Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 13, MAR 15 2019. (16/02978-6, 14/06863-3, 13/08844-3, 18/16748-8, 11/20683-0, 15/02866-0)

RUSSO, FABIELE BALDINO; FREITAS, BEATRIZ CAMILLE; PIGNATARI, GRACIELA CONCEICAO; FERNANDES, ISABELLA RODRIGUES; SEBAT, JONATHAN; MUOTRI, ALYSSON RENATO; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. Modeling the Interplay Between Neurons and Astrocytes in Autism Using Human Induced Pluripotent Stem Cells. BIOLOGICAL PSYCHIATRY, v. 83, n. 7, p. 569-578, APR 1 2018. (11/20683-0, 16/02978-6)

RUSSO, FABIELE BALDINO; BRITO, ANITA; DE FREITAS, ARIANE MIRANDA; CASTANHA, ANDRELISSA; DE FREITAS, BEATRIZ C.; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. The use of iPSC technology for modeling Autism Spectrum Disorders. Neurobiology of Disease, v. 130, OCT 2019. (11/20683-0, 16/02978-6)

BRITO, ANITA; RUSSO, FABIELE BALDINO; MUOTRI, ALYSSON RENATO; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. Autism spectrum disorders and disease modeling using stem cells. Cell and Tissue Research, v. 371, n. 1, p. 153-160, JAN 2018. (11/20683-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RUSSO, Fabiele Baldino. Generation of induced pluripotent stem cells from patients with autism spectrum disorder. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.