INVESTIGATION OF IMMUNOLOGICAL CHANGES IN MICE SUBJECTED TO ANIMAL MODEL OF SEPSIS BY CECAL LIGATION AND PUNCTURE (CLP) WITH AND WITHOUT BRAIN CHANGES

• Grant number: 12/07898-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2012
• Effective date (End): June 30, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal researcher: Francisco Garcia Soriano
• Grantee: Isabela Casagrande Jeremias
• Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 09/15530-0 - Sepsis - the role of inflammatory response: cell signaling; immune system action in sepsis; LPS tolerance and therapeutic, AP.TEM

Abstract

Sepsis is defined as the systemic inflammatory response syndrome associated with an infection and is characterized by the development of clinical, haematological, biochemical and immunological associated with infection. In this context, knowing that changes in the nervous system are implicated in the induction or amplification of the change of the immune system, or conversely, conduit, have not been well elucidated in sepsis and the importance of sepsis in the mortality found and that the interaction between bacteria and their products and innate immunity cells represent a crucial moment in the pathophysiology of inflammatory diseases, it is necessary to investigate the immune changes after changes in brains of mice in an animal model of sepsis by cecal ligation and puncture (CLP). In the present study, the animals will undergo the technique of CLP to induce sepsis, later changes brain will be evaluated by testing SHIRPA. It will be evaluated by flow cytometry profile of population of T lymphocytes (CD4, CD8, Treg, T17 and B lymphocytes) and matched in these cell lines cellular viability, apoptosis, necrosis, changes in mitochondrial membrane potential and production free radical (superoxide and nitric oxide). Furthermore, the expression is evaluated by Real time PCR apoptosis.