Influence of different doses and number of applications of low-level laser irradiation on bone repair in calvaria bone defects in Wistar rats with an experimental model of osteoporosis

• Grant number: 12/10184-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2012
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Selma Siéssere
• Grantee: Priscilla Hakime Scalize
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The bone tissue presents regenerative potential, but some diseases can lead to bone loss, such as osteoporosis, that compromised bone strength and quality, predisposing to increased risk of fractures. So, faced with this and other diseases, it becomes important to search for new methodologies that aim bone deposition. The literature has been demonstrated that the low level laser irradiation (LLLI) is a new methodology that is effective in increasing bone formation and decrease bone resorption in ovariectomized rats, but there is still no standard regarding its use, so more studies are needed to investigate the effect of different parameters such as length wave-length and number of sessions. This study aims to evaluate the effects of LLLI in critical calvaria bone defects in Wistar rats with an experimental model for osteoporosis, the quality of new bone by light microscopy and estimate the stereological parameters: total volume, volume density and surface density of trabecular neoformed bone, numerical density and total number of osteocytes and average volume of osteoblasts. The total amount of collagen will be analyzed. Fifty-four female Wistar rats will be bilaterally ovariectomized. After 150 days will be made critical bone defects in the calvaria. The rats will be divided into nine groups (n = 6) and 24 hours after making the defect will start the laser treatment (gallium aluminum arsenide- GaAlAs, 780 nm) at doses of 20 and 30 J/cm2 (3, 6 and 12 sessions). Control groups will be simulated laser application for 3, 6 and 12 sessions. The results of this project aim to contribute to understanding the biological effects of LLLI, thus contributing to science and society.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCALIZE, PRISCILLA HAKIME; DE SOUSA, LUIZ GUSTAVO; HALLAK REGALO, SIMONE CECILIO; SEMPRINI, MARISA; PITOL, DIMITRIUS LEONARDO; DA SILVA, GISELLE APARECIDA; COELHO, JESSICA DE ALMEIDA; COPPI, ANTONIO AUGUSTO; LOBO LAAD, ALINY A. B.; BOMBONATO PRADO, KARINA FITTIPALDI; et al. Low-level laser therapy improves bone formation: stereology findings for osteoporosis in rat model. Lasers in Medical Science, v. 30, n. 5, p. 1599-1607, JUL 2015. (12/10184-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SCALIZE, Priscilla Hakime. Influence of different doses and different number sessions of low-level laser irradiation in the repair of bone defects in calvaria of Wistar female rats model of osteoporosis. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.