Effects of calcium hydroxide and MTA on dental pulp stem cells with purpose of capping the regenerated dental pulp regarding gene expression

• Grant number: 12/11476-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Bruno das Neves Cavalcanti
• Grantee: Geraldo Rossi Neto
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

With the advent of tissue engineering, new strategies have been described in the literature, regarding endodontic treatment. Thus, the development or improvement of clinical therapies is necessary in order to achieve the desired success. The purpose of this study is to evaluate the biological effects of calcium hydroxide and mineral trioxide aggregate on dental pulp stem cells (DPSC), with the purpose of capping the regenerated dental pulp. DPSC will be cultured and assayed for gene expression (DSPP, DMP-1, and Runx2), at 1, 7, 14, and 21 days in contact with the materials conditioned media. Data will be compared with non-treated cells (control group).(AU)