Effects of vitamin D in the arterial hypertension and in the ischemic renal injury induced by chronic stenosis of renal artery induced by 2 Kidneys - 1 Clip model

• Grant number: 12/11607-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2012
• Effective date (End): August 31, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Mirian Aparecida Boim
• Grantee: Daniele Berruezo Lucio Silva
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Renovascular hypertension (HARV) is caused by a partial or complete obstruction of the renal artery. The experimental model of HARV most widely used was developed by Goldblatt in 1934, which consists in placing a silver clip on the left renal artery, also known as 2 kidney - 1 clip (2K-1C) model. The objective of this model is to reduce blood flow by 50% and activate the Renin-Angiotensin-Aldosterone system (RAAS), resulting in a rise in systolic blood pressure (SBP). Control of this condition is still critical, so we set out to test the hypothesis that treatment with vitamin D could be useful in minimizing the HARV based on the following criteria: 1 - The HARV is dependent on activation of the RAAS, 2 - Vit D is capable of reducing the activity of the renin gene 3 - Vit D has renoprotective effects with anti-inflammatory action. Male Wistar adult rats will be subject to partial clamping of the left renal artery. After the 3rd. week, when the pressure levels are significantly high, vitamin D will be administered at a dosage 0.5 ug/kg (ip) until the sixth week. The PAS will be monitored weekly using the plethysmography method. 24 hours urine and blood samples will be collected before and after treatment for biochemical tests. The expression of renin and other components of the RAS by western blotting and real-time PCR will be evaluated in the cortex and medulla of the clipped kidney The systemic activity of RAS will be assessed by plasma renin activity. Hematoxylin and eosin staining (HE) will be used to analyze the renal morphology. Renal function will be estimated by creatinine clearance, proteinuria, and dosage of plasma and urinary sodium and potassium.(AU)