Evaluation of polyfunctional CD4+ T cells and regulatory in the selective IgA deficiency

• Grant number: 12/14110-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Maria Notomi Sato
• Grantee: Camila de Lollo
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The selective IgA deficiency the most common primary immunodeficiency, which is susceptible to respiratory infections, gastrointestinal alterations, allergic symptoms, as well as prone to the development of autoimmune diseases. The homeostasis of the immune system depends on the balance between the innate and adaptive immune responses. The innate response recognizes the molecular patterns associated with microbial, MAMPs by Toll-like receptors (TLR), important sensors to be evaluated functionally, through the production of cytokines. Adaptive response is crucial the balance between the regulator cell subset, such as regulatory T cells, with the pro-inflammatory cells population, named Th17 cells. The aim of this project is to evaluate in selective IgA deficiency the profile of response to the agonists of TLRs and the frequency of regulatory CD4+ T cell as well as the proinflammatory/polyfunctional TCD4+ cells. This will be evaluated in patients with selective IgA deficiency compared to healthy individuals the cytokine production induced by synthetic agonists of TLRs (2-9) in peripheral blood mononuclear cells, the frequency of naïve/effector regulatory cells, CD4+ CD25+ CD127 -CD45RA-FoxP3 +, CD4 + T cells secreting IL-17a and IL-22 as well as IL-2, IL-21, IFN-³ and the determination of IL-17a, TNF-±, IFN-³ and IL-10 serum levels. These data may be indicators of the balance between innate and adaptive response, and the regulatory mechanisms and pro-inflammatory in the pathogenesis of the selective IgA deficiency.Keywords: Selective IgA, Toll-like receptors, regulatory T cells, Th17, Th22. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LOLLO, CAMILA; VASCONCELOS, DEWTON DE MORAES; DA SILVA OLIVEIRA, LUANDA MARA; DOMINGUES, ROSANA; DE CARVALHO, GABRIEL COSTA; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency. Clinical Immunology, v. 169, p. 121-127, AUG 2016. (12/14110-0)

DE LOLLO, CAMILA; VASCONCELOS, DEWTON DE MORAES; DA SILVA OLIVEIRA, LUANDA MARA; TITZ, TIAGO DE OLIVEIRA; CARNEIRO-SAMPAIO, MAGDA; ABE JACOB, CRISTINA MIUKI; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Impaired CD8(+) T cell responses upon Toll-like receptor activation in common variable immunodeficiency. JOURNAL OF TRANSLATIONAL MEDICINE, v. 14, MAY 17 2016. (12/14110-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOLLO, Camila de. Evaluation of CD4+ T regulatory and effector cells in the common variable immunodeficiency. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.