Cloning and expression of the human protein kinase Nek3 for functional, structural, molecular and cellular studies

• Grant number: 11/06730-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Jörg Kobarg
• Grantee: Vanessa Bomfim Cardoso
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil

Abstract

NEKs (NIMA-related kinases) are a group of kinase proteins that share high identity between their amino acid sequences with NIMA (Never in mitosis gene A) and are involved in cell cycle regulation. Among mammalian NEKs already studied, they are related to cilia function, in cell cycle progression, especially during checkpoints and DNA damage. Since then, eleven NEKs have been discovered, with their orthologous in murine. NEK3 is a 506-amino acid serine/threonine kinase, present in the cytoplasm and nucleus, and its gene belongs to 13q14.2 chromosome. According to the correlations between NEK3 and several diseases and processes, such as breast cancer, axonal degeneration, frequently mutations in its chromosomal site, and it have characteristics different from others best studied NEKs, it becomes necessary to further clarification of how their participation is, through functional, molecular and structural studies, aiming to map the interaction network in which it is involved. Thereunto, we will use a yeast two-hybrid assay, spectroscopic and crystallographic techniques, among other that were not done yet. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARDOSO, Vanessa Bomfim. Chracterization of the protein interaction profile of the human kinase NEK3 and assignment of its functional context. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.