Evaluation of cytotoxicity and genotoxicity of desensitizing toothpastes on human pulp fibroblasts

• Grant number: 12/00574-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2012
• End date: September 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Samira Esteves Afonso Camargo
• Grantee: Fernanda Saraiva de Oliveira
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The purpose of this study is to evaluate in vitro the possible cytotoxic and genotoxic effects of toothpaste on human pulp fibroblasts (HPF-1). It will be established four groups: Group I- desensitizing toothpaste (Colgate Sensitive Pro-alívio, Colgate-Palmolive), Group II-desensitizing toothpaste (Aquafresh Sensitive, Aquafresh®), Group III- desensitizing toothpaste (Oral B Pro Sensitive, Oral-B®)and Group IV- control (Colgate Total 12® Professional Clean, Colgate-Palmolive). The cells will be exposed to toothpaste for 24 h, and cell survival will be measured using the MTT assay in a spectrophotometer (570 nm). Genotoxicity is determined by the formation of micronuclei in cells after 24 h of exposure to toothpaste and Ethyl methanesulfonate (positive control). The differences between the rate of cell survival and a number of micronuclei will be statistically analyzed by Mann-Whitney-U (p <0.05).(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAMIRA ESTEVES AFONSO CAMARGO; NOALA VICENSOTO MOREIRA MILHAN; FERNANDA DE OLIVEIRA SARAIVA; JONATAS RAFAEL DE OLIVEIRA; LUCIANE DIAS DE OLIVEIRA; CARLOS HENRIQUE RIBEIRO CAMARGO. Are Desensitizing Toothpastes Equally Biocompatible and Effective Against Microorganisms?. Brazilian Dental Journal, v. 28, n. 5, p. 604-611, 2017-09-00. (12/00574-4)