Cytotoxicity, genotoxicity and bioactivity of different types of Portland Cement a...
| Grant number: | 18/26514-4 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | April 01, 2019 |
| Effective date (End): | November 30, 2019 |
| Field of knowledge: | Health Sciences - Dentistry |
| Principal Investigator: | Cláudio Antonio Talge Carvalho |
| Grantee: | Larissa Marques Pereira |
| Home Institution: | Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil |
Abstract Regenerative cements make up a fundamental part of the current endodontics, due to its importance in the cases of apicification, apicigenesis, pulpotomy, revascularization and others, and the mineral trioxide aggregate is the most used. The objective of this study was to evaluate the cytotoxicity and genotoxicity of the "Angelus" MTA, MTA Repair HP, five "5MO" mineral oxides and the "Ca (OH) 2" calcium hydroxide as control group. The evaluation of cytotoxicity of the cements will be performed by means of the MTT colorimetric test, which will analyze cellular mitochondrial activity after cement contact for 5 min and 24 h where cultures of human gingival fibroblasts (FMN-1) and mouse macrophages ( RAW 264.7). In addition, the micronucleus test will be performed to evaluate the genotoxicity according to the guideline of OECD 2016 (TG487) for the 2 cell lines: fibroblasts and macrophages. The data will be analyzed statistically by the ANOVA method complemented by Tukey's test, with a significance level of 5% (pd0.05). | |