Taxonomic characterization of mycobacteria belonging to Mycobacterium chelonae-M.abscessus group without conclusive species identification

• Grant number: 12/13763-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2012
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Sylvia Luisa Pincherle Cardoso Leão
• Grantee: Christiane Lourenço Nogueira
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 11/18326-4 - Mycobacteria and extrachromosomal elements: molecular characterization and biotechnological applications, AP.TEM
• Associated scholarship(s): 14/00746-5 - Molecular analysis of Mycobacterium chelonae-M. abscessus group isolates without conclusive species identification, BE.EP.DR

Abstract

Although ubiquitous environmental organisms, nontuberculous mycobacteria can cause opportunistic infections in humans, especially the species belonging to M. chelonae-M. abscessus group. Until recently, this group was composed of M. chelonae, M. abscessus, M. immunogenum M. bolletii, M. massiliense and M. salmoniphilum. Taxonomic changes in this group have been proposed including the unification of M. abscessus, M. massiliense e M. bolletii in a single species (M. abscessus) with two subspecies (M. abscessus subsp. abscessus and M. abscessus subsp. bolletii) and the inclusion of Mycobacterium franklinii in M. chelonae-M. abscessus group. Several commercial kits are available for rapid mycobacteria identification such as INNO-LiPA and Genotype. The first one separates the mycobacteria of M. chelonae-M. abscessus group in clusters (CHI, CHIII e CHII/CHIV). Considering the importance of correct species identification in this group for outbreak surveillance and clinical management of patients, this project aims to analyze M. chelonae-M. abscessus group isolates with inconclusive species classification. Moreover, this project also aims to analyze the taxonomic classification of a collection of CHII cluster isolates. The results of this work will contribute to epidemiological analysis and future outbreaks surveillance. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA, CHRISTIANE LOURENCO; WHIPPS, CHRISTOPHER M.; MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; DROZ, SARA; TORTOLI, ENRICO; DE FREITAS, DENISE; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; et al. Mycobacterium saopaulense sp nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, n. 12, p. 4403-4409, DEC 2015. (12/13763-0, 11/18326-4, 13/16018-6)

NOGUEIRA, CHRISTIANE L.; DE ALMEIDA, LUIZ G. P.; MENENDEZ, MARIA C.; GARCIA, MARIA J.; DIGIAMPIETRI, LUCIANO A.; CHIMARA, ERICA; CNOCKAERT, MARGO; PALOMINO, JUAN C.; PORTAELS, FRANCOISE; MARTIN, ANANDI; et al. Characterization of Mycobacterium chelonae-Like Strains by Comparative Genomics. FRONTIERS IN MICROBIOLOGY, v. 8, MAY 8 2017. (12/13763-0, 11/18326-4)

MATSUMOTO, CRISTIANNE KAYOKO; MARTINS BISPO, PAULO JOSE; SANTIN, KATIANE; NOGUEIRA, CHRISTIANE LOURENCO; LEAO, SYLVIA CARDOSO. Demonstration of Plasmid-Mediated Drug Resistance in Mycobacterium abscessus. Journal of Clinical Microbiology, v. 52, n. 5, p. 1727-1729, MAY 2014. (12/13763-0, 11/18326-4)

NASCIMENTO, HELOISA; VIANA-NIERO, CRISTINA; NOGUEIRA, CHRISTIANE LOURENCO; MARTINS BISPO, PAULO JOSE; PINTO, FERNANDO; PEREIRA UZAM, CAMILA DE PAULA; MATSUMOTO, CRISTIANNE KAYOKO; OLIVEIRA MACHADO, ANTONIA MARIA; LEAO, SYLVIA CARDOSO; HOFLING-LIMA, ANA LUISA; et al. Identification of the Infection Source of an Outbreak of Mycobacterium Chelonae Keratitis After Laser in Situ Keratomileusis. CORNEA, v. 37, n. 1, p. 116-122, JAN 2018. (12/13763-0, 13/16018-6, 11/18326-4)

NOGUEIRA, CHRISTIANE LOURENCO; WHIPPS, CHRISTOPHER M.; MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; DROZ, SARA; TORTOLI, ENRICO; DE FREITAS, DENISE; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; et al. Mycobacterium saopaulense sp nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, p. 7-pg., 2015-12-01. (12/13763-0, 11/18326-4, 13/16018-6)

NOGUEIRA, CHRISTIANE LOURENCO; SIMMON, KEITH E.; CHIMARA, ERICA; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; VANDAMME, PETER; BROWN-ELLIOTT, BARBARA A.; WALLACE, JR., RICHARD J.; LEAO, SYLVIA CARDOSO. Mycobacterium franklinii sp nov., a species closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, n. 7, p. 2148-2153, JUL 2015. (12/13763-0, 11/18326-4)

NOGUEIRA, CHRISTIANE LOURENCO; SIMMON, KEITH E.; CHIMARA, ERICA; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; VANDAMME, PETER; BROWN-ELLIOTT, BARBARA A.; WALLACE, RICHARD J., JR.; LEAO, SYLVIA CARDOSO. Mycobacterium franklinii sp nov., a species closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, p. 6-pg., 2015-07-01. (12/13763-0, 11/18326-4)