Advanced search
Start date
Betweenand

Validation of miRNAs and mRNAs-target interactions involved in nervous system development in female embryos of Apis mellifera

Grant number: 12/02061-4
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 16, 2012
Effective date (End): July 15, 2013
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Zilá Luz Paulino Simões
Grantee:Flávia Cristina de Paula Freitas
Supervisor abroad: Charles Claudianos
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: University of Queensland, Brisbane (UQ), Australia  
Associated to the scholarship:10/08332-4 - Transcriptome analysis of embryonic development in Apis mellifera females, BP.DR

Abstract

Based on regulatory function of miRNAS, the aim of Flávia's PhD project (FAPESP Process No. 2010/08332-4) is identify interactions between microRNAs and target RNAs that control female embryonic development of Apis mellifera. Thus, we built mRNAs and small RNAs libraries for five embryonic developmental phases: clivage, blastula, gastrulation, germ-band extension and retraction, and larval body formation. Three hundred and eighteen miRNAs-target interactions were suggested by antagonistic profiles of miRNAs and mRNAs and by miRNAs binding-sites in 3' UTR of targets. Thus, the next step is to validate interactions in which genes involved in nervous system development take part. First, we would like to refine target prediction by comparing miRNA-target interactions found in our data with other insect species. Then, we would like to experimentally validate miRNA-targets using a luciferase reporter system in S2 cells. Validation experiments will be conducted in collaboration with Charles Claudianos and Alexandre dos Santos, both researchers from Queensland Brain Institute (Brisbane, Austrália). This is justified by the history of collaboration with these researchers, the experience they have with analyses of genomic sequences conserved, and the Queensland Brain Institute infrastructure that will allow us realize validation experiments. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREITAS, FLAVIA C. P.; PIRES, CAMILLA V.; CLAUDIANOS, CHARLES; CRISTINO, ALEXANDRE S.; SIMOES, ZILA L. P. MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee. SCIENTIFIC REPORTS, v. 7, JAN 18 2017. Web of Science Citations: 5.
CRISTINO, ALEXANDRE S.; BARCHUK, ANGEL R.; FREITAS, FLAVIA C. P.; NARAYANAN, RAMESH K.; BIERGANS, STEPHANIE D.; ZHAO, ZHENGYANG; SIMOES, ZILA L. P.; REINHARD, JUDITH; CLAUDIANOS, CHARLES. Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee. NATURE COMMUNICATIONS, v. 5, NOV 2014. Web of Science Citations: 31.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.