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Study of the role of PLIN2 in inflammatory response induced by MT-III, a Phospholipase A2 isolated from Bothrops asper snake venom: lipid bodies biogenesis and release of inflammatory mediators

Grant number: 12/06730-8
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): August 10, 2012
Effective date (End): February 09, 2013
Field of knowledge:Biological Sciences - Pharmacology
Principal Investigator:Catarina de Fatima Pereira Teixeira
Grantee:Elbio Leiguez Junior
Supervisor abroad: Jesús Balsinde
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Local de pesquisa : Universidad de Valladolid (UVa), Spain  
Associated to the scholarship:10/06345-1 - Study of factors involved in lipid bodies formation induced by a Phospholipase A2, isolated from Bothrops asper snake venom: synthesis and metabolism of lipids, BP.DR

Abstract

Bothrops venoms contain high concentrations of secreted phospholipases A2 (sPLA2) which have structural and functional homology with mammalian PLA2s, whose levels are increased in inflammatory diseases such as atherosclerosis. We have recently demonstrated that MT-III, a sPLA2 group IIA, induced an increase in lipid body formation and expression of adipofilina (ADRP) or PLIN2, a protein involved in lipid metabolism and related to the accumulation of lipids in lipid bodies. However, the role of PLIN2 in the inflammatory response induced by MT-III has not been elucidated. Thus, this study aims to evaluate the effects of MT-III in macrophages depleted for PLIN2, with emphasis on factors involved in triggering the inflammatory response. The aim of this study is to assess the role of PLIN2 in the inflammatory effects induced by the snake venom sPLA2 MT-III in depleted macrophages to PLIN2, such as: 1) lipid body formation, 2) release of the lipid mediators (PGE2, PGD2, LTB4) and proinflammatory cytokines (TNF-alfa, IL-1B and IL-6) 3) arachidonic acid incorporation into triacylglycerol. This work will contribute to understanding the role of PLIN2 the inflammatory response induced by MT-III, a secreted phospholipases A2, and intracellular processes that regulate the accumulation of lipids in immunocompetent cells, in addition to better understanding of the pathophysiology of local reaction caused by Bothrops venoms. Still, this work may show new therapeutic targets in diseases in which the inflammatory response and lipid imbalance are triggers factors. (AU)

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