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Episodic-like memory and oscillatory patterns in the hippocampus and medial prefrontal cortex of adult rats subjected to early-life seizures

Grant number: 12/07107-2
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal researcher:João Pereira Leite
Grantee:Cleiton Lopes Aguiar
Supervisor abroad: Eva Marie Carlén
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: University of Edinburgh, Scotland  

Abstract

Several clinical and experimental studies have provided convincing evidence showing that early-life seizure (ELS) events trigger late spontaneous seizures and/or cognitive deficits; however, the underlying mechanism of such late changes remains unclear. Neuronal cell loss and mossy fibre sprouting may be absent in the presence of later seizures and cognitive deficits. On the other hand, inflammatory process associated to microglia over-activation, besides plasticity-related events at a molecular/cellular level, usually occurs not only in the hippocampus but probably also in extra-limbic circuits. One possibility to probe neuronal plasticity on the network level in vivo is quantifying oscillatory activity patterns of local field potentials (LFPs). Therefore, further studies are required using freely moving electrophysiological recordings throughout an animal's life and attendant memory tasks in order to dissociate different phases and facets of the episodic-like memory processing. In this sense, the event arena paradigm represents an opportunity to investigate the effect of ELS into adulthood, specifically with respect to (1) the temporal dynamics of systems memory consolidation, and (2) the hippocampus/medial prefrontal cortex (mPFC) encoding responsible for enabling relevant new paired associates (PA; flavour/place) to be assimilated into an original schema. Therefore, the aim of the present project is, in adult animals previously subjected to ELS, to investigate the relationship between oscillatory activity in hippocampus and mPFC with the cognitive performance of an episodic-like memory task. In particular, we aim to verify whether the inhibition of ELS-associated inflammation process by minocycline treatment attenuates the later oscillatory effects, likewise later learning ability impairments in the event-arena. Rats will be subjected to ELS induction by Li-PILO at P12 and seven additional daily injections of minocycline. Control groups will be treated only with its drugs vehicles. Forty-eight days after ELS, electrodes in the mPFC and in CA1 of the hippocampus will be stereotaxically implanted under anaesthesia. Rats will be subjected to the event arena task, LFPs will be recorded daily using a telemetry system and eventual cellular loss and changes in microglia will be examined. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos 
Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders 
Científicos emplean la ingeniería genética para entender los mecanismos de los trastornos psiquiátricos 
Articles published in other media outlets (44 total):
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UOL: Engenharia genética é usada para investigar transtornos psiquiátricos (28/Apr/2021)
M3 India (Índia): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (15/May/2021)
Enggtalks (Índia): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (14/May/2021)
Medindia (Índia): Genetic Engineering Helps Explore Details of Psychiatric Disorders (13/May/2021)
Programa InfoSalud (Argentina): Científicos emplean la ingeniería genética para entender los mecanismos de los trastornos psiquiátricos (13/May/2021)
MDLinx (EUA): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (13/May/2021)
Nonur - Health News: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (13/May/2021)
News O Time (Estados Unidos): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (13/May/2021)
India News Republic: Genetic Engineering Helps Explore Details of Psychiatric Disorders (13/May/2021)
Detonic: Wetenschappers gebruiken genetische manipulatie om mechanismen te onderzoeken die betrokken zijn bij psychiatrische stoornissen (13/May/2021)
Ingentium News Magazine: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (13/May/2021)
Bioengineer (Reino Unido): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Science Codex: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Scienmag Science Magazine (Reino Unido): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
InfoSurHoy (EUA): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders. (12/May/2021)
7thSpace: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Health News: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
INS - Infusion Nurses Society (EUA): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Polymerelektronik.org: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
LaptrinhX: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
California News Times Today (EUA): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Press-news.org: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Mental Fitness Group: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
MediExpose (ìndia): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
4State News: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Newscon: Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Newspostalk (EUA): Scientists use genetic engineering to discover mechanisms concerned in psychiatric issues (12/May/2021)
Jan Academy: Scientists use genetic engineering to discover mechanisms concerned in psychiatric issues (12/May/2021)
Zephyrnet.com (China): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Zephyrnet.com (Espanha): Scientists use genetic engineering to explore mechanisms involved in psychiatric disorders (12/May/2021)
Na Balança NF: Engenharia genética é usada para investigar transtornos psiquiátricos (10/May/2021)
Revista Sustinere online: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (06/May/2021)
Plantão News (MT): Pesquisadores usam engenharia genética para investigar mecanismos em transtornos psiquiátricos (02/May/2021)
Diário do Pará online: Pesquisadores brasileiros criam vírus para estudar o cérebro (29/Apr/2021)
Belém online: Pesquisadores brasileiros criam vírus para estudar o cérebro (29/Apr/2021)
Blog Jornal da Mulher: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Saense: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Portal da Enfermagem: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Programa InfoSalud (Argentina): Investigadores utilizan ingeniería genética para investigar mecanismos involucrados en trastornos psiquiátricos (28/Apr/2021)
Diario de Ciencias Aplicadas (Argentina): Crean Un Virus Benigno. Ingeniería Genética en Psiquiatría. Las Interneuronas (28/Apr/2021)
Novo Cantu Notícias: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Revista Cenarium online: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Quimiweb: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)
Guia Viver Bem: Pesquisadores usam engenharia genética para investigar mecanismos envolvidos em transtornos psiquiátricos (28/Apr/2021)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUYON, NICOLAS; ZACHARIAS, LEONARDO RAKAUSKAS; VAN LUNTEREN, JOSINA ANNA; IMMENSCHUH, JANA; FUZIK, JANOS; MARTIN, ANTJE; XUAN, YANG; ZILBERTER, MISHA; KIM, HOSEOK; MELETIS, KONSTANTINOS; LOPES-AGUIAR, CLEITON; CARLEN, MARIE. Adult trkB Signaling in Parvalbumin Interneurons is Essential to Prefrontal Network Dynamics. JOURNAL OF NEUROSCIENCE, v. 41, n. 14, p. 3120-3141, APR 7 2021. Web of Science Citations: 2.
GUYON, NICOLAS; ZACHARIAS, LEONARDO RAKAUSKAS; DE OLIVEIRA, ELIEZYER FERMINO; KIM, HOSEOK; LEITE, JOAO PEREIRA; LOPES-AGUIAR, CLEITO; CARLEN, MARIE. Network Asynchrony Underlying Increased Broadband Gamma Power. JOURNAL OF NEUROSCIENCE, v. 41, n. 13, p. 2944-2963, MAR 31 2021. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.