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Effects of resveratrol and lithium on lipid profile oxidative stress and apoptosis. study of the protective role in senescent rats

Grant number: 12/13206-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2012
Effective date (End): September 30, 2014
Field of knowledge:Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
Principal Investigator:Soraya Soubhi Smaili
Grantee:Katiucha Karolina Honório Ribeiro Rocha
Home Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). São Paulo , SP, Brazil


Aging is associated with the development of metabolic disorders and neurodegenerative diseases triggered by the decrease of antioxidant defenses and alterations in the apoptotic and autophagic processes. It is known that resveratrol and lithium can be used to protect from cell degeneration. However, the mechanisms involved in these effects and the benefits related to the association of the two agents in senescence have not been studied yet. The main of this study is to investigate the the mechanisms related to resveratrol, resveratrol and the association lithium-lithium in the neuroprotection of the striatum and protection of liver in young and senescent animals. For this purpose, there will be two groups used for treatment : the young (4-6 months old) and old group (22-26 months old) of female rats Young animals will be divided into four subgroups (n = 5): C group, control that will receive distilled water ; R group, which will receive resveratrol solution (6 mg / L); L group, which will receive lithium (standard concentration used for neuroprotection); RL group, will receive resveratrol solution plus lithium . The old group will be divided into four subgroups (n = 5): S group, which will receive distilled water; SR group which will receive resveratrol solution (6 mg / L); the SL, which will receive lithium; SRL-group which will receive resveratrol and lithium. After 30 days of experiments, the animals will be euthanized to collect the blood, liver and striatum. We will analyze glucose, lipid profile in serum, as well as apoptosis, autophagy, mitochondrial viability and oxidative stress of the liver and striatum. The antioxidant activity of resveratrol combined with autophagy induced by lithium will be investigated and the possible protective of the increase in autophagy activity, which may potentiate the antioxidants effects, to prevent oxidative stress, mitochondrial damage and cell degeneration associated with the aging process.