"Interaction between canine dental pulp stem cells and canine osteosarcoma"

• Grant number: 11/11982-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2012
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Maria Angelica Miglino
• Grantee: Dayane Alcântara
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 13/19866-8 - miRNAs and mRNA Molecular Profile of Mestatatics and Non Metastatics Osteosarcomas, BE.EP.DR

Abstract

Osteosarcoma is a common malignant bone tumor in dogs, with a preference for large breeds, and has a high metastatic potential. The diagnosis is based on clinical history, physical examination, radiological and histopathological findings. Treatment consists of tumor resection and choice protocol is amputation associated with chemotherapy. Osteosarcoma has similarities in dogs and humans, thus canine osteosarcoma can be a useful model for studying this disease in humans. Currently, stem cells studies increased due to their high therapeutic potential and they can also secrete factors that modulate tumor growth. The aim of this study was to evaluate cell interaction, proliferation or inhibition potential, and gene expression involved in the canine osteosarcoma treatment with canine dental pulp stem cells. Canine dental pulp stem cells will be grown in Alpha MEM culture medium supplemented with 10% of serum HyClone, antibiotics (1%), non-essential amino acids (1%) and L-glutamine (1%). Osteosarcoma cells will be grown in DMEM-H, supplemented with 10% bovine fetal serum, antibiotics (1%) and sodium pyruvate (1%). The treatment will be performed in a transwell coculture system using canine dental pulp stem cells in the upper well and osteosarcoma cells in the lower well. Biochemical analyses will be evaluated by ELISA. The cell morphology will be evaluated by inverted microscopy. Markers expression, cell death pathways, cell cycle and mitochondrial electrical potential will be analyzed by flow cytometry. Abnormalities in gene expression will be evaluated by qRTPCR.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALCÂNTARA, Dayane. Interaction between immature dental pulp stem cells and canine osteosarcoma. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.