The involvement of nitric oxide synthase inducible (iNOS) on the endoplasmic reticulum stress in obesity induced by high fat diet

• Grant number: 12/15009-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2012
• Effective date (End): September 30, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal researcher: Mario Jose Abdalla Saad
• Grantee: Tamires Marques Zanotto
• Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The major aim of this study is investigate the role of iNOS in endoplasmic reticulum stress, using mice with selective deletion of the gene encoding iNOS fed a high-fat diet for 3 months. Additionally, this effect will be evaluated in animals fed high-fat diet and treated with a selective blocker of iNOS. Thus, we will investigate the role of iNOS on insulin sensitivity through the insulin tolerance test and hyperinsulinemic-euglycemic CLAMP, and the expression/phosphorylation of insulin signaling pathway and endoplasmic reticulum stress proteins. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CELESTRIN, CAROLINA P.; ROCHA, GUILHERME Z.; STEIN, ANGELICA M.; GUADAGNINI, DIOZE; TADELLE, RAFAEL M.; SAAD, MARIO J. A.; OLIVEIRA, ALEXANDRE G. Effects of a four week detraining period on physical, metabolic, and inflammatory profiles of elderly women who regularly participate in a program of strength training. European Review of Aging and Physical Activity, v. 17, n. 1 AUG 26 2020. Web of Science Citations: 0.

BAGAROLLI, RENATA A.; TOBAR, NATALIA; OLIVEIRA, ALEXANDRE G.; ARAUJO, TIAGO G.; CARVALHO, BRUNO M.; ROCHA, GUILHERME Z.; VECINA, JULIANA F.; CALISTO, KELLY; GUADAGNINI, DIOZE; PRADA, PATRICIA O.; SANTOS, ANDREY; SAAD, SARA T. O.; SAAD, MARIO J. A. Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 50, p. 16-25, DEC 2017. Web of Science Citations: 32.

ZANOTTO, TAMIRES M.; QUARESMA, PAULA G. F.; GUADAGNINI, DIOZE; WEISSMANN, LAIS; SANTOS, ANDRESSA C.; VECINA, JULIANA F.; CALISTO, KELLY; SANTOS, ANDREY; PRADA, PATRCIA O.; SAAD, MARIO J. A. Blocking iNOS and endoplasmic reticulum stress synergistically improves insulin resistance in mice. MOLECULAR METABOLISM, v. 6, n. 2 FEB 2017. Web of Science Citations: 10.