CELLULAR AND MOLECULAR ANALYSIS OF TUMOR CELLS IN RESPONSE TO THE EFFECT OF CHALCONE

• Grant number: 12/15862-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2012
• End date: September 30, 2014
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Ana Lucia Fachin Saltoratto
• Grantee: Luis Felipe Buso Bortolotto
• Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil

Abstract

In the context of mammalian health, the cancer is among the most important diseases in terms of occurrence rates, especially in humans. Neoplasias, besides the genetic causes, are also a consequence of harmful habits. Thus, the urgency need for selective and nontoxic anticancer drugs, against the mechanisms of multidrug resistance, is critical. This is not only interesting for science, but for the economy. The pharmaceutical industry tends to prioritize the researches for untapped activities of known compounds from the nature, particularly the phytochemicals. Many plant secondary metabolites have apoptosis-related cytotoxicity, which is intriguing to pharmacology. Currently, the evolution of therapy emphasized the polyphenols inhibition of topoisomerases, carcinogenic markers. Among these flavonoids, chalcones stand out. In the present work, the cytotoxicity, genotoxicity, inhibition of topoisomerases and pro-apoptotic potential by chalcones will be evaluated in one healthy and five tumor cell lines, through MTT, LDH and AO/EB staining tests, comet assay and gene expression analysis. The aim of this study is to improve the existing knowledge from an underexplored approach, providing information about different cell lines, thereby contributing to the development of formulations for prevention and/or reversal of cancer.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOMOTO, TATIANA TAKAHASI; BERNARDES, TAYNA MINERVINA; MESQUITA, THAIS BALTHAZAR; BUSO BORTOLOTTO, LUIS FELIPE; SILVA, GABRIEL; BITENCOURT, TAMIRES APARECIDA; BAEK, SEUNG JOON; MARINS, MOZART; FACHIN, ANA LUCIA. Chalcones Repressed the AURKA and MDR Proteins Involved in Metastasis and Multiple Drug Resistance in Breast Cancer Cell Lines. Molecules, v. 23, n. 8, AUG 2018. (16/22701-9, 15/23435-8, 12/15862-5)

BUSO BORTOLOTTO, LUIS FELIPE; BARBOSA, FLAVIA REGINA; SILVA, GABRIEL; BITENCOURT, TAMIRES APARECIDA; BELEBONI, RENE OLIVEIRA; BAEK, SEUNG JOON; MARINS, MOZART; FACHIN, ANA LUCIA. Cytotoxicity of trans-chalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest. BIOMEDICINE & PHARMACOTHERAPY, v. 85, p. 425-433, JAN 2017. (12/06889-7, 12/02920-7, 12/15862-5)