Influence of pulpal infection alone or associated with diabetes in the quantification of proinflammatory cytokine IL-17 in periapical lesions and organs of rats.

• Grant number: 12/02565-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2012
• End date: April 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Mariane Maffei Azuma
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This study will evaluate the influence of pulpal infection alone or associated with diabetes in the quantification of proinflammatory cytokine IL-17 in periapical lesions and organs of rats. Will be used 40 mice divided into four groups of 10 animals: G1 - normal rats, G2 - rats with periapical lesion, G3 - diabetic rats, G4 - diabetic rats with pulpal infection. Diabetes is induced by application of streptozotocin intravenously and confirmed in 24/48 hours subsequent evaluation by glucose. The periapical lesion is induced by exposure of the maxillary right first molar to the oral environment. After 30 postoperative days the animals will be sacrificed. The heart, liver and kidneys of all animals, as well as periapical lesions of groups 2 and 4 are removed. Will be used the ELISA to quantify the proinflammatory cytokine IL-17 in periapical lesions in animal bodies. The results are analyzed and compared in terms of statistical significance level of 5% (p <0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZUMA, MARIANE MAFFEI; GOMES-FILHO, JOAO EDUARDO; CARRARA PRIETO, ANNELISE KATRINE; SAMUEL, RENATA OLIVEIRA; FELIX DE LIMA, VALERIA MARCAL; SUMIDA, DORIS HISSAKO; ERVOLINO, EDILSON; ANGELO CINTRA, LUCIANO TAVARES. Diabetes increases interleukin-17 levels in periapical, hepatic, and renal tissues in rats. ARCHIVES OF ORAL BIOLOGY, v. 83, p. 230-235, NOV 2017. (12/02565-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AZUMA, Mariane Maffei. Influência da infecção endodôntica associada ou não à diabetes nos níveis da citocina pró-inflamatória IL-17 nos tecidos periapical, hepático e renal de ratos Wistar. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.