Local and systemic influence of dietary supplementation with omega - 3 in rats with endodontic infection

• Grant number: 13/26390-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2014
• End date: August 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Mariane Maffei Azuma
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 15/07107-0 - The possible anti-bone formation role of semaforina 4D expressed in the context of periapical periodontitis, BE.EP.DR

Abstract

Introduction: Previous studies from our research group (FAPESP Process No. 2012/02083-8 and 2012/02565-2; Prope-UNESP 005 /2011) demonstrated in an animal model that endodontic infection exerts effects on systemic health as well as certain systemic changes can modulate the development of endodontic infection (Cintra et al, 2013abc; 2014ab). Thus, we believe it is important to study therapies that modulate the process of bone resorption resulting from endodontic infection, in order to minimize its systemic effects. Objective: The objective of this work is locally and systemically evaluate the influence of endodontic infection in rats supplemented or not supplemented with omega-3. Methods: Will be used 40 rats divided into 4 groups of 10 animals each: C - control rats, C+S - rats supplemented with omega-3 ; IE - rats with endodontic infection; IE+S - rats with endodontic infection supplemented with omega-3. The omega-3 supplementation will be taken by oral gavage for 15 days before induction of endodontic infections and 30 days after induction, total of 45 days of supplementation. The endodontic infection is induced by exposure of the pulp first and second molars of the upper and lower right side of each animal. After 30 days, will be collected the blood, kidney, liver, submandibular lymphnodes, the jaws and the jaws Hematology tissue as well as organs will be used for quantification of proinflammatory cytokines IL -17, TNF-±, IL -6 and OC protein binding. Furthermore, the blood profile will be traced. Histological and histometric analyzes of periapical lesions will be performed, as well as immunohistochemical staining for proinflammatory cytokines IL-17, TNF-± and IL-6 and OC protein. Analysis of results: The results of the different analyzes and the correlation between local and systemic findings will be analyzed by specific statistical tests for each case (p<0.05).

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZUMA, MARIANE MAFFEI; EDUARDO GOMES-FILHO, JOAO; ERVOLINO, EDILSON; MORAES CARDOSO, CAROLINA DE BARROS; PIPA, CAMILA BARBOSA; KAWAI, TOSHIHISA; CONTI, LETICIA CITELLI; ANGELO CINTRA, LUCIANO TAVARES. Omega-3 Fatty Acids Reduce Inflammation in Rat Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 44, n. 4, p. 604-608, APR 2018. (13/26390-0)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; DA SILVA, CRISTIANE CANTIGA; DE OLIVEIRA, PEDRO HENRIQUE CHAVES; JACINTO, ROGERIO DE CASTILHO; ANDRADA, ANA CRISTINA; CINTRA, LUCIANO TAVARES ANGELO. The use of omega-3 fatty acids in the treatment of oral diseases. ORAL DISEASES, v. 28, n. 2, p. 11-pg., 2020-10-27. (15/03054-0, 13/26390-0)

MARIANE MAFFEI AZUMA; JOÃO EDUARDO GOMES-FILHO; CAROLINA DE BARROS MORAIS CARDOSO; CAMILA BARBOSA PIPA; LUIS GUSTAVO NARCISO; SUELY REGINA MOGAMI BOMFIM; ROGÉRIO DE CASTILHO JACINTO; LUCIANO TAVARES ANGELO CINTRA. Omega 3 Fatty Acids Reduce the Triglyceride Levels in Rats with Apical Periodontitis. Brazilian Dental Journal, v. 29, n. 2, p. 173-178, 2018-03-00. (13/26390-0, 15/03054-0)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; DA SILVA, CRISTIANE CANTIGA; DE OLIVEIRA, PEDRO HENRIQUE CHAVES; JACINTO, ROGERIO DE CASTILHO; ANDRADA, ANA CRISTINA; CINTRA, LUCIANO TAVARES ANGELO. he use of omega-3 fatty acids in the treatment of oral disease. ORAL DISEASES, v. 28, n. 2, OCT 2020. (13/26390-0, 15/03054-0)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; SAMUEL, RENATA OLIVEIRA; PIPA, CAMILA BARBOSA; MOGAMI BOMFIM, SUELY REGINA; NARCISO, LUIS GUSTAVO; GOMES-FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES. Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 47, n. 2, p. 272-277, FEB 2021. (15/03054-0, 13/26390-0)

AZUMA, MARIANE MAFFEI; GOMES-FILHO, JOAO EDUARDO; ERVOLINO, EDILSON; PIPA, CAMILA BARBOSA; MORAIS CARDOSO, CAROLINA DE BARROS; ANDRADA, ANA CRISTINA; KAWAI, TOSHIHISA; ANGELO CINTRA, LUCIANO TAVARES. Omega 3 Fatty Acids Reduce Bone Resorption While Promoting Bone Generation in Rat Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 43, n. 6, p. 970-976, JUN 2017. (15/03054-0, 13/26390-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AZUMA, Mariane Maffei. Local and systemic influence of the dietary supplement with omega 3 fatty acids in rats with endodontic infection. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp).