Introduction: Previous studies from our research group (FAPESP Process No. 2012/02083-8 and 2012/02565-2; Prope-UNESP 005 /2011) demonstrated in an animal model that endodontic infection exerts effects on systemic health as well as certain systemic changes can modulate the development of endodontic infection (Cintra et al, 2013abc; 2014ab). Thus, we believe it is important to study therapies that modulate the process of bone resorption resulting from endodontic infection, in order to minimize its systemic effects. Objective: The objective of this work is locally and systemically evaluate the influence of endodontic infection in rats supplemented or not supplemented with omega-3. Methods: Will be used 40 rats divided into 4 groups of 10 animals each: C - control rats, C+S - rats supplemented with omega-3 ; IE - rats with endodontic infection; IE+S - rats with endodontic infection supplemented with omega-3. The omega-3 supplementation will be taken by oral gavage for 15 days before induction of endodontic infections and 30 days after induction, total of 45 days of supplementation. The endodontic infection is induced by exposure of the pulp first and second molars of the upper and lower right side of each animal. After 30 days, will be collected the blood, kidney, liver, submandibular lymphnodes, the jaws and the jaws Hematology tissue as well as organs will be used for quantification of proinflammatory cytokines IL -17, TNF-±, IL -6 and OC protein binding. Furthermore, the blood profile will be traced. Histological and histometric analyzes of periapical lesions will be performed, as well as immunohistochemical staining for proinflammatory cytokines IL-17, TNF-± and IL-6 and OC protein. Analysis of results: The results of the different analyzes and the correlation between local and systemic findings will be analyzed by specific statistical tests for each case (p<0.05).
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