| Grant number: | 12/16143-2 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Start date: | November 01, 2012 |
| End date: | October 31, 2015 |
| Field of knowledge: | Biological Sciences - Morphology - Anatomy |
| Principal Investigator: | Carlos Alberto Vicentini |
| Grantee: | Claudemir Kuhn Faccioli |
| Host Institution: | Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil |
Abstract The knowledge of morphological characteristics of the digestive tract, associated with the processes of digestion and absorption, it is essential to understanding the physiology and nutrition of fish, assisting in planning diets to provide better management and success in farming. Thus, the aim of this study is to analyze the ultrastructural and cytochemical features of the digestive tract of adult specimen of Hemisorubim platyrhynchos to support cultivation in fish farms and preservation of the species. H. platyrhynchos is a carnivorous specie, distributed in the basins of the Orinoco, Amazonas, Paraguai, Uruguai and Paraná. This fish presents great potential for consumption, due to the animal size and meat quality. It is also used for ornamental fish due to the staining pattern of this species. However, this species is endangered in the São Paulo state, with reduced inventory and isolated populations. For this, thirty specimens will be killed and dissected to remove fragments of the esophagus (initial, medial and final portion), stomach (cardic, fundic and pyloric regions) and intestine (anterior, middle, posterior and rectum). For ultrastructural studies, fragments will be fixed in modified Karnovsky solution, post-fixed in osmium tetroxide and destinated for inclusion in the transmission electron microscopy routine. For cytochemical studies, tissue samples will be fixed on appropriate solutions and destined for the following cytochemical techniques: impregnation with zinc iodide-osmium tetroxide - ZIO (targeting analysis of membranous organelles), method osmium-imidazole (to detect of lipid droplets), method for detection of alkaline and acid phosphatase and method for detection of acid (ruthenium red) and neutral (periodic acid-thiosemicarbazide-silver proteinate) glycoconjugates. These techniques enable a detailed analysis of the cellular characteristics of the epithelial lining, aiding in the understanding of cell physiology along the digestive tract of H. platyrhynchos. (AU) | |
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