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Ultrastructure and cytochemistry of the digestive tract of Hemisorubim platyrhynchos (Teleostei, Siluriformes, Pimelodidae)

Grant number: 12/16143-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2015
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Carlos Alberto Vicentini
Grantee:Claudemir Kuhn Faccioli
Home Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil

Abstract

The knowledge of morphological characteristics of the digestive tract, associated with the processes of digestion and absorption, it is essential to understanding the physiology and nutrition of fish, assisting in planning diets to provide better management and success in farming. Thus, the aim of this study is to analyze the ultrastructural and cytochemical features of the digestive tract of adult specimen of Hemisorubim platyrhynchos to support cultivation in fish farms and preservation of the species. H. platyrhynchos is a carnivorous specie, distributed in the basins of the Orinoco, Amazonas, Paraguai, Uruguai and Paraná. This fish presents great potential for consumption, due to the animal size and meat quality. It is also used for ornamental fish due to the staining pattern of this species. However, this species is endangered in the São Paulo state, with reduced inventory and isolated populations. For this, thirty specimens will be killed and dissected to remove fragments of the esophagus (initial, medial and final portion), stomach (cardic, fundic and pyloric regions) and intestine (anterior, middle, posterior and rectum). For ultrastructural studies, fragments will be fixed in modified Karnovsky solution, post-fixed in osmium tetroxide and destinated for inclusion in the transmission electron microscopy routine. For cytochemical studies, tissue samples will be fixed on appropriate solutions and destined for the following cytochemical techniques: impregnation with zinc iodide-osmium tetroxide - ZIO (targeting analysis of membranous organelles), method osmium-imidazole (to detect of lipid droplets), method for detection of alkaline and acid phosphatase and method for detection of acid (ruthenium red) and neutral (periodic acid-thiosemicarbazide-silver proteinate) glycoconjugates. These techniques enable a detailed analysis of the cellular characteristics of the epithelial lining, aiding in the understanding of cell physiology along the digestive tract of H. platyrhynchos. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FACCIOLI, C. K.; CHEDID, R. A.; MORI, R. H.; DO AMARAL, A. C.; VICENTINI, I. B. F.; VICENTINI, C. A. Cytochemical features of the digestive tract mucosa of Hemisorubim platyrhynchos (Siluriformes: Pimelodidae). Journal of Fish Biology, v. 92, n. 6, p. 1915-1928, JUN 2018. Web of Science Citations: 1.
FACCIOLI, CLAUDEMIR KUHN; CHEDID, RENATA ALARI; MORI, RICARDO HIDEO; DO AMARAL, ANTONIO CARLOS; FRANCESCHINI VICENTINI, IRENE BASTOS; VICENTINI, CARLOS ALBERTO. Ultrastructure of the digestive tract in neotropical carnivorous catfish Hemisorubim platyrhynchos (Valenciennes, 1840) (Siluriformes, Pimelodidae). SCANNING, v. 38, n. 4, p. 336-343, JUL-AUG 2016. Web of Science Citations: 1.
FACCIOLI, CLAUDEMIR KUHN; CHEDID, RENATA ALARI; MORITA, RICARDO HIDEO; DO AMARAL, ANTONIO CARLOS; FRANCESCHINI-VICENTINI, IRENE BASTOS; VICENTINI, CARLOS ALBERTO. Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos. ACTA HISTOCHEMICA, v. 118, n. 7, p. 722-728, 2016. Web of Science Citations: 4.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FACCIOLI, Claudemir Kuhn. Ultraestrutura e citoquímica do tubo digestivo de Hemisorubim platyrhynchos (Teleostei, Siluriformes, Pimelodidae). 2015. 100 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências, Letras e Ciências Exatas..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.