Influence of different irrigating solutions associated to photodynamic therapy: in vitro cytotoxicity study in fibroblasts L-929

• Grant number: 12/12695-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2012
• End date: October 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Nayara Kívilla Do Carmo Maia
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Photodynamic therapy (PDT) is based on appropriate physical, chemical and biological effects, which occurs when the photosensitizer (FS) is activated by light of a specific wavelength to destroy the target cell and/or micro-organisms. The aim of this study is to evaluate the in vitro cytotoxicity of PDT compared with irrigating solutions. The cell line used be of mouse fibroblast L-929. The cultures are maintained under standard cell culture (370 C com 5% de CO2). The solutions will be distributed in the following groups: G1-2.5% sodium hypochlorite; G2-5% sodium hypochlorite; G3-2% chlorhexidine; G4-0.9% sodium chloride; G5-PDT (FS curcumin activated by blue light). Fibrin sponge will be inserted into polyethylene tubes sterile. The sponges are embedded in 0.1 ml of each solution to be tested for 24h, 48h, 96h, and 144h. Six wells are used for each material and empty tubes are used as controls. The results of cell viability will be conducted by the MTT colorimetric method. The solution is added to each well containing the fibroblasts which remain incubated for 4h at (370 C com 5% de CO2). Isopropyl alcohol is added to the cells, to dissolve the formazan crystals. The solution will be taken to the spectrophotometer with a wavelength of 570 nm for absorbance reading. The results will be statistically analyzed by ANOVA with Bonferroni correction (p<0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES-FILHO, JOAO EDUARDO; SIVIERI-ARAUJO, GUSTAVO; SIPERT, CARLA RENATA; DA SILVA SANTOS, LUDMILLA MOTA; DE AZEVEDO QUEIROZ, INDIA OLINTA; MARTINS, CHRISTINE MEN; DO CARMO MAIA, NAYARA KIVILLA; ANGELO CINTRA, LUCIANO TAVARES; DEZAN-JUNIOR, ELOI; BAGNATO, VANDERLEI SALVADOR; et al. Evaluation of photodynamic therapy on fibroblast viability and cytokine production. Photodiagnosis and Photodynamic Therapy, v. 13, p. 97-100, MAR 2016. (12/06785-7, 11/23287-8, 12/12695-0)