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Influence of different irrigating solutions associated to photodynamic therapy: study in rats

Grant number: 12/12696-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2013
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:João Eduardo Gomes Filho
Grantee:Fernanda Esteves
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Photodynamic therapy (PDT) is based on appropriate physical, chemical and biological effects, which occurs when the photosensitizer (FS) is activated by light of specific wavelength to destroy the target cell and/or micro-organisms. However, the literature is scarce on the biocompatibility of PDT. The aim of this study is to evaluate the tissue response of PDT by implanting polyethylene tubes in rats, compared with irrigating solutions. Fifty rats (Wistar), between 4 and 6 months of age, weighing between 250 g and 280g were distributed in: G1-2.5% sodium hypochlorite; G2-5% sodium hypochlorite; G3-2% chlorhexidine; G4-0.9% sodium chloride; G5-PDT (FS curcumin activated by blue light). Following anesthesia, are inserted fibrin sponge embedded in polyethylene tubes containing the solutions mentioned above. After 7, 15, 30, 60 and 90 days of implantation period, the animals are were killed. and the polyethylene tubes to the surrounding tissues are removed and fixed in 10% formalin solution, pH 7.0. Polyethylene tubes are cut, processed and embedded in glycol methacrylate (GMA), sectioned in serial sections of 3 ¼m and 10 ¼m thick, respectively stained by H.E. and Von Kossa, or remain to be examined without staining under polarized light. The reactions of the tissue in contact with the material at the opening of polyethylene tubes will be evaluated. Scores will be determined according to the histological results and statistically analyzed by Kruskal-Wallis test (p<0.05).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIVIERI-ARAUJO, GUSTAVO; DE AZEVEDO QUEIROZ, INDIA OLINTA; DAL FABBRO, RENAN; ESTEVES, FERNANDA; ANGELO CINTRA, LUCIANO TAVARES; TOBIAS DUARTE, PAULO CARVALHO; BAGNATO, VANDERLEI SALVADOR; PENHA OLIVEIRA, SANDRA HELENA; GOMES-FILHO, JOAO EDUARDO. Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318, JUN 2017. Web of Science Citations: 3.

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