Citokynes and inflamatory receptors of primary dermic human fibroblasts in burned patients

• Grant number: 12/18689-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2012
• End date: October 31, 2013
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alfredo Gragnani Filho
• Grantee: Leonardo Monteiro
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

It is known that a burn on the skin triggers an inflammatory response resulting from alterations in gene expression. An effective method to assess which genes change during the acute phase of inflammation is the PCR array. Therefore, the hypothesis was developed using this method in burn patients (large and small) in order to build a profile of genes activated in each group of patients to guide clinical interventions possible and allow the development of future studies to enable better prospects for the clinical treatment of major burn patients in the acute phase. For the survey, three patient groups will be formed: large burn patients, small burn patients, and control patients. The main difference between the first two groups is the burned body surface area (5% in the small burned and from 25 to 50% in the large burned). Thus, the objective is to evaluate 84 genes involved in inflammation by PCR array. The genes are extracted from dermic fibroblasts from burned human skin. Finally, it is expected to give an overview of the genes up-regulated and down-regulated and then build a network of signs to guide effective therapy.(AU)