Amino acids, polyamines and nitric oxide as modulators of zygotic and somatic polyembryogenesis in Araucaria angustifolia (Bert) O. Ktze.

• Grant number: 12/51145-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2012
• End date: November 30, 2015
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Eny Iochevet Segal Floh
• Grantee: André Luiz Wendt dos Santos
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Embryogenesis is a complex, highly organized biological process that plays a central role in the life cycle of an organism. More specifically for gymnosperms, zygotic and somatic polyembryogenesis (SP) models have been proven to be promising tools in elementary studies on cell biology, physiology and biochemistry as well as on large-scale vegetative propagation and the establishment of in vitro germplasm banks. Although clonal propagation and ex situ conservation of elite genotypes based on SP is now a Consolidated method for a number of gymnosperm species, the adoption of suboptimal conditions during in vitro growth of somatic polyembryos has restricted its use in Araucaria angustifolia. One of the approaches developed to correctly handle in vitro embryos is based on comparative investigations between zygotic and somatic polyembryogenesis. In line with previous studies conducted in BIOCEL/IB/USP on signaling molecules and morphogenesis, the present research project was conceived to investigate the role of modulation of amino acids, polyamines and nitric oxide during zygotic and somatic polyembryogenesis of A. angustifolia. These studies will pave the way for a more in-depth understanding of essential aspects of the plant cell differentiation processes in vitro and in situ, and generate relevant information for the development of efficient somatic embryogenesis protocols for conifers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WENDT DOS SANTOS, ANDRE LUIS; ELBL, PAULA; NAVARRO, BRUNO VIANA; DE OLIVEIRA, LEANDRO FRANCISCO; SALVATO, FERNANDO; BALBUENA, TIAGO SANTANA; SEGAL FLOH, ENY IOCHEVET. Quantitative proteomic analysis of Araucaria angustzfolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential. JOURNAL OF PROTEOMICS, v. 130, p. 180-189, JAN 1 2016. (14/26888-0, 12/51145-6)