Polyamine metabolism in zygotic and somatic embryogenesis of Araucaria angustifolia (Bert.) O. Kuntze

• Grant number: 12/22738-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2013
• End date: January 31, 2017
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Eny Iochevet Segal Floh
• Grantee: Leandro Francisco de Oliveira
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Araucaria angustifolia is a native conifer species of Brazil. In function of its intensive exploitation, the species occupies only 2% of its natural vegetation. In this system, biotechnology techniques, like somatic embryogenesis, although restricted to early stages of somatic embryo development, may be integrated into breeding and conservation programs. The similarity between zygotic and somatic embryogenesis has allowed comparative studies that provide a better understanding of developmental processes and their optimization for application as biotechnological technique. Polyamine metabolism, specifically putrescine, spermidine and spermine, has demonstrated as fundamental for the comprehension and evolution of zygotic and somatic embryogenesis. However, the biosynthesis and catabolism pathway for this substances are unknown in conifers. Thus, the aim of this project is the study of polyamine metabolism during zygotic and somatic embryogenesis of A. angustifolia. For this, it will be carried out studies of enzymatic activity and gene expression of two enzymes related to polyamine biosynthesis (arginine decarboxylase and ornithine decarboxylase) during zygotic and somatic embryo development. In addition, amino acids levels will be evaluated and their presence will be correlated with polyamine concentration in zygotic and somatic embryos. Results will allow a better comprehension of polyamine metabolism in A. angustifolia, during process involving embryo formation as well as it will provide essential information for the establishment of efficient protocols for somatic embryogenesis in conifers.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, LEANDRO FRANCISCO; NAVARRO, BRUNO VIANA; CERRUTI, GIOVANNI VICTORIO; ELBL, PAULA; MINOCHA, RAKESH; MINOCHA, SUBHASH C.; WENDT DOS SANTOS, ANDRE LUIS; SEGAL FLOH, ENY IOCHEVET. Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential. Plant and Cell Physiology, v. 59, n. 5, p. 1084-1098, MAY 2018. (14/26888-0, 15/21075-4, 12/22738-9)

DE OLIVEIRA, LEANDRO FRANCISCO; DOS SANTOS, ANDRE LUIS WENDT; FLOH, ENY IOCHEVET SEGAL. Polyamine and amino acid profiles in immature Araucaria angustifolia seeds and their association with embryogenic culture establishment. TREES-STRUCTURE AND FUNCTION, v. 34, n. 3, DEC 2019. (15/21075-4, 12/22738-9, 16/17541-2, 17/01284-3, 18/08215-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Leandro Francisco de. Polyamine metabolism in zygotic and somatic embryogenesis of Araucaria angustifolia (Bertol.) Kuntze. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.