Immunocytogenetic analysis in the cichlid fish Astatotilapia latifasciata: epigenetic inferences and its contributions in understanding the biological role of B chromosomes

• Grant number: 12/21546-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: June 30, 2017
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: Adauto Lima Cardoso
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 16/07743-7 - Experimental validation of candidate microRNAs as regulators of tRNA methylation genes over effect of b chromosomes and sex, BE.EP.DR

Abstract

B chromosomes have been identified in several groups of eukaryotes and are traditionally considered as accessory elements that do not influence the phenotype of their hosts, so they are dispensable. However, this vision has been changed because some recent works suggest possible transcriptional activity of these chromosomes and possible advantage to the host organism. Cichlid fishes have been used with model in genetic and genomic studies and the accumulation of information has provided important contributions in the knowledge of structure and genomic evolution. The cichlid Astatotilapia latifasciata became target of investigations about the possible biological effect of B chromosomes via studies involving structural and functional genomics. However, studies on epigenetic modifications of the chromatin and its interaction with specific factors, which interfere in the genic expression, still need to be conducted. A significant way of focus in epigenomic analyzes is the use of antibodies that recognize proteins or protein modifications present in the chromatin (immunocytogenetics). The present project have the aim to advance in understanding the possible effects of B chromosome presence in the organisms, based in the specific epigenetics patterns of this chromosome using A. latifasciata as a model and immunocytogenetic tools. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, ADAUTO LIMA; DE ALMEIDA FANTINATTI, BRUNO EVARISTO; VENTURELLI, NATALIA BORTHOLAZZI; CARMELLO, BIANCA DE OLIVEIRA; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the Cichlid Astatotilapia latifasciata. FRONTIERS IN GENETICS, v. 10, APR 12 2019. (15/16661-1, 13/04533-3, 12/21546-9)

CARDOSO, ADAUTO LIMA; DE ALMEIDA FANTINATTI, BRUNO EVARISTO; VENTURELLI, NATALIA BORTHOLAZZI; CARMELLO, BIANCA DE OLIVEIRA; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the Cichlid Astatotilapia latifasciata. FRONTIERS IN GENETICS, v. 10, p. 14-pg., 2019-04-12. (12/21546-9, 13/04533-3, 15/16661-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARDOSO, Adauto Lima. Effects of B chromosomes in the regulation of gene expression in the cichlid Astatotilapia latifasciata. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.