PHENOTIPIC AND GENOTIPIC IDENTIFICATION OF MICRO-ORGANISMS IN BLOOD CULTURES OF PATIENTS IN INTENSIVE CARE UNIT: IMPLICATIONS AND PROFILE PATHOGENIC COAGULASE-NEGATIVE Staphylococcus.

• Grant number: 12/15366-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Maria de Lourdes Ribeiro de Souza da Cunha
• Grantee: Aydir Cecilia Marinho Monteiro
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The detection of bacteremia by examination of blood culture remains one of the most important functions of the clinical microbiology laboratory, to be able to elucidate causes of disease and guide appropriate antibiotic treatment to improve the prognosis of patients with sepsis, reducing morbidity and mortality. Even with automation, detection of microorganisms in the blood, its identification and determination of the profile of its antimicrobial susceptibility are still time-consuming processes, so methods are needed faster, more efficient, especially when it comes to the urgency inherent in cases of bacteremia. The automated system VITEK 2 is a device capable of automatically perform all the steps necessary for the identification and determination of antimicrobial susceptibility after a primary inoculum prepared and standardized. This system is shown apparently a promising technique for bacterial identification due to its speed, accuracy and sensitivity in obtaining the results so far published in existing studies. Many bacteria are isolated from the bloodstream and aerobic gram-positive cocci exceeded gram-negative bacilli as major etiologic agents of bacteremia in the hospital environment, in most cases in patients kept in intensive care units (ICUs) and is central to distinction between contamination and infection, as these microorganisms are prevalent in the skin and can contaminate blood cultures during the collection. This study will be conducted in two stages: first will aim to compare the performance of species identification systems (manual and automated) for microorganisms isolated from blood cultures. The second step will be the prospective study design. At this stage, patients with blood cultures positive for coagulase-negative staphylococci will be monitored over time to identify clinical factors and virulence of these microorganisms associated with the prognosis.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MONTEIRO, Aydir Cecilia Marinho. Identification of microorganisms present in blood cultures of patients in intensive care units and evaluation of coagulase-negative Staphylococcus. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.