Efficacy and toxicity of low-temperature plasma on pathogenic biofilms developed in in vitro reconstituted human epithelium

• Grant number: 12/20699-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Carlos Eduardo Vergani
• Grantee: Juliana Aparecida Delben
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 13/21388-7 - Effect of low-temperature plasma on pathogenic oral biofilms and in vitro reconstituted oral epithelium, BE.EP.PD

Abstract

Staphylococcus aureus and Candida albicans are responsible for multi-species oral biofilms with severe virulence in patients with inefficient immunological system, mainly in association with methicillin-resistant S. aureus (MRSA). In this sense, low-temperature plasma (LTP) is a promising approach against microorganisms due to disruption of biofilm matrix and rupture of cell structure. However, there is a lack of studies reporting the effect of LTP on multi-species biofilms and host tissue. So, the aim of this study is to evaluate the antimicrobial effect and toxicity of LTP on single- and multi-species biofilm with C. albicans, methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in in vitro reconstituted human epithelium. The biofilms will be cultured and submitted to LTP treatment for evaluation of viable colonies, total biomass, cellular mitochondrial activity, laser confocal microscopy (LCM) and environmental scanning electron microscopy (ESEM). Similar treatment will be applied on non-infected in vitro reconstituted human epithelium and analyzed through histological analysis, immunohistochemistry, lactate dehydrogenase release (LDH), inflammatory indicators, LCM and ESEM. The reconstituted oral tissue will be infected with single- and multi-species cultures and submitted to LTP treatment. Microbial invasion will be evaluated under histological analysis, immunohistochemistry, LDH release, inflammatory indicators, LCM and ESEM. The control group will be characterized by no LTP treatment for each experimental condition. Data will be evaluated through qualitative and statistical methods. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DELBEN, JULIANA APARECIDA; ZAGO, CHAIENE EVELIN; TYHOVYCH, NATALIA; DUARTE, SIMONE; VERGANI, CARLOS EDUARDO. Effect of Atmospheric-Pressure Cold Plasma on Pathogenic Oral Biofilms and In Vitro Reconstituted Oral Epithelium. PLoS One, v. 11, n. 5, MAY 25 2016. (12/20699-6, 13/21388-7)