Study of biocompatibility of different magnetic nanoparticles in cervical cancer cell line

• Grant number: 12/22809-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2013
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Marilia de Freitas Calmon
• Grantee: Ana Beatriz Bortolozo de Oliveira
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

The nanotherapeutic applied to cancer treatment is evolving rapidly and it is being implemented in order to solve the limitations of conventional therapeutic strategies. Such nanocomposites have demonstrated advantages over other carriers due to their nanoscale structure with unique properties, being uncoated or coated with different polymers, including nanoscale structures such as iron oxides. Its applications range from drug delivery to procedures involving hyperthermia. However, its use may be limited by causing alterations in cell morphology, mitochondrial function, plasma membrane permeability, or triggering cell apoptosis. The aim of this study is to investigate if the new nanoparticles synthesized from Fe3O4 magnetic nanoparticle iron with replacement of ion Fe by other ions (CoFe2O4, NiFe2O4, and Fe2O4 Zn) show alterations in cellular internalization and biocompatibility compared to Fe3O4 nanoparticle, which has been used as a diagnostic tool and is being tested for treatment by hyperthermia against cancer. To ensure biocompatibility, the interaction of these nanoparticles with the cells will be investigated by MTT assay, Acridine Orange (apoptosis), and Prussian Blue staining. This work will be able to demonstrate the potential benefits of these new nanoparticles in future hyperthermia treatment of an important tumor type in order to arouse academic interest in this innovative and promising field. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORTOLOZO OLIVEIRA, ANA BEATRIZ; DE MORAES, FABIO ROGERIO; CANDIDO, NATALIA MARIA; SAMPAIO, ISABELLA; PAULA, ALEX SILVA; DE VASCONCELLOS, ADRIAN; SILVA, THAIS CERQUEIRA; MILLER, ALEX HENRIQUE; RAHAL, PAULA; NERY, JOSE GERALDO; et al. Metabolic Effects of Cobalt Ferrite Nanoparticles on Cervical Carcinoma Cells and Nontumorigenic Keratinocytes. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 12, p. 4337-4348, DEC 2016. (09/53989-4, 12/22809-3)