|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||March 01, 2011|
|Effective date (End):||February 28, 2013|
|Field of knowledge:||Biological Sciences - Genetics - Human and Medical Genetics|
|Principal Investigator:||Paula Rahal|
|Grantee:||Natalia Maria Candido|
|Home Institution:||Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil|
The nanotherapy applied to cancer treatment is evolving rapidly and new techniques are being implemented in order to solve limitations of conventional therapeutic strategies. Such nanocomposites have demonstrated advantages over other carriers because of their nanoscale structure with unique properties, being whether or not covered by different polymers and including nanoscale structures such as iron oxides. Their applications extend from the delivery of drugs to procedures involving hyperthermia. However, their use may be limited since can cause changes in cell morphology, mitochondrial function, plasma membrane permeability or initiate the process of apoptosis. The purpose of this study is to investigate the cytotoxicity of the magnetic iron nanoparticles in cell lines of oral cancer, their effects on components of the cell medium and their action in vivo. To ensure biocompatibility, the interactions of these nanostructures with different cell lines will be investigated by UV spectroscopy, MTT assay, apoptosis assay and light and electron microscopies. In addition, animal experiments will be employed using Syrian hamsters to evaluate the therapeutic profile of nanoparticles coated with PVA when combined with hyperthermia. Thus, the study will address the therapeutic potential of these nanocomposites with in vitro and in vivo tests, to improve the current biomedical approaches and understanding of the cellular and systemic mechanisms of toxicity of these newly synthesized and potentially commercial magnetites.