Enzymatic production of N-acetyl-glucosamine by Aeromonas sp. isolated from marine ecosystem

• Grant number: 12/16824-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Adalberto Pessoa Junior
• Grantee: Flávio Augusto Cardozo
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Chitinolytic bacteria are widely distributed in the marine environment and are extremely important in the process of degradation of chitin. The degradation of chitin is performed by enzymes called chitinases, which have different functions in nature and can be applied in various fields, especially in the production of derivatives of chitin. The production of N-acetyl-glucosamine has attracted attention in recent years due to its features and has great potential to be used as a component in cosmetics and for bioethanol production. This derivative is traditionally produced by chemical hydrolysis of chitin. However, the procedures used in these processes generate losses and produce large amounts of chemical residues that can affect the environment. Thus, application of bacterial chitinases as an alternative to chemical hydrolysis of chitin can be very viable, since the bacteria are easier to handle, have lower production costs, are susceptible to genetic engineering and contribute to the self-sustainable development. Furthermore, the use of these enzymes in the production process would avoid the use of chemicals that may cause environmental damage. Given this and the knowledge acquired since 2005 with chitinolytic bacteria in the laboratory of molecular microbial ecology, it is essential to explore the ability of the isolates studied in the Masters for the production of N-acetyl-glucosamine. Thus, this study aims to evaluate the production of chitinases and N-acetyl-glucosamine in bioreactors by a chitinolytic bacteria selected of the Aeromonas genus isolated from marine ecosystem. The bacteria selected will be characterized as to species level by MLST (Multilocus Sequence Type) using eight housekeeping genes (gyrB, groL, gltA, metG, ppsA, recA, rpoB and rpoD), as the metabolic capacity through two biochemical identification systems and seven for the presence of genes associated with virulence (ahh1, asa1, cur, ast, ascV, eno, and aexT). More detailed tests will be used to assess the chitinases expression levels and determine the enzymes expressed in bioreactor.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOZO, FLAVIO AUGUSTO; ZIMPEL, CRISTINA KRAEMER; SA GUIMARAES, ANA MARCIA; PESSOA, ADALBERTO; GUTIERREZ RIVERA, IRMA NELLY. Draft Genome Sequence of Marine-Derived Aeromonas caviae CHZ306, a Potential Chitinase Producer Strain. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 6, NOV-DEC 2016. (12/16824-0, 13/18773-6)

CARDOZO, FLAVIO AUGUSTO; FACCHINATTO, WILLIAM MARCONDES; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO; PESSOA, ADALBERTO. Bioproduction of N-acetyl-glucosamine from colloidal alpha-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 35, n. 8, AUG 2019. (16/20970-2, 12/16824-0, 13/18773-6)

CARDOZO, FLVIO AUGUSTO; MIGUEL GONZALEZ, JUAN; FEITOSA, VALKER ARAUJO; PESSOA, ADALBERTO; GUTIERREZ RIVERA, IRMA NELLY. Bioconversion of alpha-chitin into N-acetyl-glucosamine using chitinases produced by marine-derived Aeromonas caviae isolates. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 33, n. 11, NOV 2017. (13/18773-6, 12/16824-0)

CARDOZO, FLAVIO AUGUSTO; ZIMPEL, CRISTINA KRAEMER; SA GUIMARAES, ANA MARCIA; PESSOA, ADALBERTO; GUTIERREZ RIVERA, IRMA NELLY. Draft Genome Sequence of Marine-Derived Aeromonas caviae CHZ306, a Potential Chitinase Producer Strain. GENOME ANNOUNCEMENTS, v. 4, n. 6, p. 2-pg., 2016-11-01. (13/18773-6, 12/16824-0)

CARDOZO, FLAVIO AUGUSTO; FEITOSA, VALKER; MENDONCA, CARLOS MIGUEL NOBREGA; DA SILVA, FRANCISCO VITOR SANTOS; CONVERTI, ATTILIO; OLIVEIRA, RICARDO PINHEIRO DE SOUZA; PESSOA JR, ADALBERTO. Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor. Brazilian Journal of Microbiology, v. N/A, p. 13-pg., 2023-08-23. (13/18773-6, 12/16824-0)

CARDOZO, FLAVIO AUGUSTO; ALFONSO VARGA, NADIA CATALINA; ZIMPEL, CRISTINA KRAEMER; PESSOA, ADALBERTO; GUTIERREZ RIVERA, IRMA NELLY. Draft Genome Sequence of Aeromonas caviae CH129, a Marine-Derived Bacterium Isolated from the Coast of Sao Paulo State, Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 6, NOV-DEC 2016. (13/18773-6, 12/16824-0)

CARDOZO, FLAVIO AUGUSTO; ALFONSO VARGA, NADIA CATALINA; ZIMPEL, CRISTINA KRAEMER; PESSOA, ADALBERTO; GUTIERREZ RIVERA, IRMA NELLY. Draft Genome Sequence of Aeromonas caviae CH129, a Marine-Derived Bacterium Isolated from the Coast of Sao Paulo State, Brazil. GENOME ANNOUNCEMENTS, v. 4, n. 6, p. 1-pg., 2016-11-01. (13/18773-6, 12/16824-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARDOZO, Flávio Augusto. Enzymatic production of N-acetyl-glucosamine by Aeromonas sp. isolated from the marine ecosystem.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.