Chitinolytic bacteria are widely distributed in the marine environment and are extremely important in the process of degradation of chitin. The degradation of chitin is performed by enzymes called chitinases, which have different functions in nature and can be applied in various fields, especially in the production of derivatives of chitin. The production of N-acetyl-glucosamine has attracted attention in recent years due to its features and has great potential to be used as a component in cosmetics and for bioethanol production. This derivative is traditionally produced by chemical hydrolysis of chitin. However, the procedures used in these processes generate losses and produce large amounts of chemical residues that can affect the environment. Thus, application of bacterial chitinases as an alternative to chemical hydrolysis of chitin can be very viable, since the bacteria are easier to handle, have lower production costs, are susceptible to genetic engineering and contribute to the self-sustainable development. Furthermore, the use of these enzymes in the production process would avoid the use of chemicals that may cause environmental damage. Given this and the knowledge acquired since 2005 with chitinolytic bacteria in the laboratory of molecular microbial ecology, it is essential to explore the ability of the isolates studied in the Masters for the production of N-acetyl-glucosamine. Thus, this study aims to evaluate the production of chitinases and N-acetyl-glucosamine in bioreactors by a chitinolytic bacteria selected of the Aeromonas genus isolated from marine ecosystem. The bacteria selected will be characterized as to species level by MLST (Multilocus Sequence Type) using eight housekeeping genes (gyrB, groL, gltA, metG, ppsA, recA, rpoB and rpoD), as the metabolic capacity through two biochemical identification systems and seven for the presence of genes associated with virulence (ahh1, asa1, cur, ast, ascV, eno, and aexT). More detailed tests will be used to assess the chitinases expression levels and determine the enzymes expressed in bioreactor.
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