Rational design of multifunctional enzymes for depolimerization of lignocelulosic material

• Grant number: 12/24147-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: May 03, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Richard John Ward
• Grantee: Lara Aparecida Buffoni de Campos Carneiro
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 10/18850-2 - Identification, characterization and engineering of plant cell wall degrading enzymes, AP.TEM
• Associated scholarship(s): 16/15708-7 - Characterization of novel hydrolysis products from the synergistic hydrolysis of xyloglucan by a beta-galactosidase/alpha-xylosidase mixture, BE.EP.DR

Abstract

In the face of growing energy demand, the need for diversification of sources of renewable energy is clear. The production of first generation bioethanol relies on technologies that utilize either sucrose from sugarcane or starch from seeds, resulting in increased presuure on arable land and instability of food prices. Thus, technologies based on the depolymerization of abundant lignocellulosic material for production of second generation bioethanol appear promising. Lignocellulosic materials represent the most significant fraction of plant biomass and they are composed mainly of cellulose, hemicellulose and lignin. Microbial enzymes play a key role in the degradation of lignocellulose, in which polymeric and/or oligosaccharidic derivatives can be used to obtaining biotechnological products with higher commercial value. Therefore, technologies aimed at cell wall saccharification are the target of worldwide research. However, hydrolysis of the complex cell wall structure is a major challenge, and the use of enzyme chimera involving specific enzyme combinations represents a promising way to complete the hydrolysis of cell wall polysaccharides, in addition to providing reduced cost of using enzymes in industrial processes. In this project we propose the construction and characterization of chimeric enzymes from alpha-xylosidase, beta-galactosidase and xiloglucanase, which are involved in the degradation of hemicellulosic polysaccharide xyloglucan.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARNEIRO, LARA A. B. C.; YU, LI; DUPREE, PAUL; WARD, RICHARD J.. Characterization of a beta-galactosidase from Bacillus subtilis with transgalactosylation activity. International Journal of Biological Macromolecules, v. 120, n. A, p. 279-287, DEC 2018. (12/24147-8, 10/18850-2)

CARNEIRO, LARA A. B. C.; WARD, RICHARD J.. Functionalization of paramagnetic nanoparticles for protein immobilization and purification. Analytical Biochemistry, v. 540, p. 45-51, JAN 1 2018. (10/18850-2, 16/15708-7, 12/24147-8)

BUFFONI DE CAMPOS CARNEIRO, LARA APARECIDA; FUZO, CARLOS ALESSANDRO; MELEIRO, LUANA PARRAS; CARLI, SIBELI; BARRETO, MATHEUS QUINTANA; LOURENZONI, MARCOS ROBERTO; BUCKERIDGE, MARCOS SILVEIRA; WARD, RICHARD JOHN. Selective xyloglucan oligosaccharide hydrolysis by a GH31 alpha-xylosidase from Escherichia coli. Carbohydrate Polymers, v. 284, p. 12-pg., 2022-05-15. (12/24147-8, 17/13734-3, 16/24139-6, 16/17582-0, 14/50884-5, 16/15708-7)

BUFFONI DE CAMPOS CARNEIRO, LARA APARECIDA; WURMAN, JOEL; DUPREE, PAUL; WARD, RICHARD JOHN. The Glycoside Hydrolase Family 35 beta-galactosidase from Trichoderma reesei debranches xyloglucan oligosaccharides from tamarind and jatoba. Biochimie, v. 211, p. 9-pg., 2023-02-27. (14/50884-5, 16/24139-6, 12/24147-8, 16/15708-7)

CARNEIRO, LARA A. B. C.; YU, LI; DUPREE, PAUL; WARD, RICHARD J.. Characterization of a beta-galactosidase from Bacillus subtilis with transgalactosylation activity. International Journal of Biological Macromolecules, v. 120, p. 9-pg., 2018-12-01. (12/24147-8, 10/18850-2)

SOARES, JESSICA M.; CARNEIRO, LARA A. B. C.; BARRETO, MATHEUS Q.; WARD, RICHARD J.. Co-immobilization of multiple enzymes on ferromagnetic nanoparticles for the depolymerization of xyloglucan. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 16, n. 6, p. 14-pg., 2022-08-02. (16/24139-6, 17/14452-1, 14/50884-5, 12/24147-8, 16/15708-7)