| Grant number: | 12/24418-1 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | March 01, 2013 |
| End date: | June 30, 2016 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine |
| Principal Investigator: | Luciana Correia de Almeida Regitano |
| Grantee: | Daniela Dantas Moré |
| Host Institution: | Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil |
Abstract Ticks are haematophagous parasite of domestic and wild animals and potential vector of many diseases. Many efforts have been done to control this parasite once that they impair substantially the animal production. Unfortunately, tick control has been done by the use of acaricides that contaminate the environment and animal products, and also induce tick resistance. Researching on alternatives of control has indicated that genetic resistance of the host is the best choice against this parasite. Additionally, the development of new generation technologies to analysis genomic data and the sequencing of the bovine genome bring the unique opportunity to perform genomic association studies (GWAS) and marked assistedselection (MAS) in animal breeding. In this way, the present study aims to analyze differential gene expression against tick infestation in 40 Hereford bovines presenting contrasting phenotypes, e.g. the 20 most and the 20 less infested animals, among more than 8000 counts performed. For this, mRNA extractedfrom infested and non infested cattle skin will be submitted to sequencing (RNA-Seq) in Illumina platform. High-throughput bioinformatic tools will be used to detect SNPs in transcribed (QTLs) and regulatory (eQTLs) regions, alternative transcription sites and imprinted genes to draw a map of the skin transcriptomein response to tick infestation. Additionally, the data obtained will be integrated with the results of the GWAS performed with the same animals to help the search of candidate genes, the pathways analysis and to draw metabolic maps. The differences observed in the genetic mechanisms against tick infestation in Herefordcattle certainly will contribute to the genomic selection of this trait. | |
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