Mechanisms of infertility caused by gossipol in rats and protective effect of vitamin E

• Grant number: 12/08668-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2013
• End date: March 31, 2014
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Fábio Erminio Mingatto
• Grantee: Andréia Tieme de Santana
• Host Institution: Universidade Estadual Paulista (UNESP). Campus Experimental de Dracena. Dracena , SP, Brazil

Abstract

The bran cotton (Gossypium sp) is the world's second most important source available or protein supplement for animal feed, surpassed only by soybeans. Gossypol is a toxic phenolic substance present in cottonseed that has been shown to reduce fertility in farm animals. In this sense, it is essential to elucidate the toxicological characteristics of gossypol, so that cottonseed meal can be used safely by farmers without harming animal performance. Due to these aspects the aim of this work is to evaluate the effects of gossypol on sperm production in rats including damage assessment in homogenate and mitochondria isolated from testes, besides analyzing the action of vitamin E in inhibiting the toxic effects of gossypol. Will be used 4 groups of 6 animals each, namely G1 (control) animals treated with canola oil; G2 (TG): will receive solution of gossypol Acetic Acid dissolved in canola oil as a vehicle (10 mg/kg body weight); G3 (TE): will receive solution containing vitamin E dissolved in canola oil as a vehicle (100 mg/kg body weight); G4 (TG + E) will receive solution of gossypol Acetic Acid (10 mg/kg body weight) and solution containing vitamin E (100 mg/kg body weight). The treatment will last for two weeks and at the end will be performed the analyze of sperm concentration, activity of the enzymes glutathione peroxidase (GPx) and glutathione reductase (GR), determining levels of GSH and GSSG, evaluation of lipid peroxidation and determination of protein thiol groups in testis homogenate, and respiration, membrane potential and ATP synthesis in mitochondria isolated from testis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, ANDREIA T.; GUELFI, MARIELI; MEDEIROS, HYLLANA C. D.; TAVARES, MARCO A.; BIZERRA, PAULO F. V.; MINGATTO, FABIO E.. Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E. Biological Research, v. 48, JUL 31 2015. (12/08668-8, 12/22684-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTANA, Andréia Tieme de. Mecanismos da infertilidade causada por Gossipol em ratos e efeito protetor da vitamina E. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira Ilha Solteira.