Immune response induced in mice by transcutaneous immunization with recombinant leptospira Lip L32 protein

• Grant number: 12/25176-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2013
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Waldely de Oliveira Dias
• Grantee: Pamela Siumey Liu
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Annually, there are more than 500,000 cases of leptospirosis worldwide and its incidence is directly related to low socioeconomic status and disability in basic sanitation services, being a matter of great importance for public health. Leptospirosis is acquired by contact with the urine of animals carrying the disease through broken skin or mucosa, by ingestion of contaminated food or water and contact with contaminated soils. This study will assess the immune response induced by transcutaneous immunization of mice with recombinant LipL32 leptospira protein compared to subcutaneous immunization. The application of antigen will be made after pretreatment of the immunization site, with emollients: pulmonary surfactant produced by Butantan or polyethylene glycol (PEG). After the immunization schedule it will be evaluate the humoral and secretory immune responses in the immunized animals and controls, as well as the cellular immune response, by measurement of cytokines in splenocytes "in vitro" stimulated with the antigen.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIU, Pamela Siumey. Immune response induced in mice by transcutaneous immunization with recombinant protein LipL32 of leptospira.. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.