| Grant number: | 12/24408-6 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | April 01, 2013 |
| End date: | November 30, 2016 |
| Field of knowledge: | Biological Sciences - Morphology - Anatomy |
| Principal Investigator: | Marucia Chacur |
| Grantee: | Joyce Teixeira da Silva |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated scholarship(s): | 14/20983-1 - Analysis of brain changes by magnetic resonance imaging after chronic pain in rodents, BE.EP.DD |
Abstract The analysis of magnetic resonance imaging called "pain matrix", i.e. brain areas related to pain have attracted great interest from the scientific community, since the range of animal models of pain facilitation and predictive data to provide a therapeutic agents vast knowledge central nervous system. The nerve growth factor (NGF) is a neurotrophin that is closely linked to the induction and maintenance of painful processes. Being the main factor related to increased substance P, an important mediator of nociception. Anti-NGF treatment is a search for various painful pathologies, however, its participation in chronic neuropathic pain with the analysis of magnetic resonance imaging and immunogold labeling for Fos is unknown. In order to elucidate the mechanisms involved in both the model of peripheral neuropathy and post-treatment with anti-NGF will be used adult male Wistar rats subjected to induction of neuropathic pain by sciatic nerve surgery. False-operated animals submitted the same incision that operated animals, but without nerve ligation and naive animals without any surgical intervention, will be used as control. After the fourteenth day, the animals are treated with anti-NGF or vehicle. To assess the nociceptive threshold will be determined mechanical hyperalgesia, thermal hyperalgesia and allodynia before any procedure after induction of neuropathy and during treatment. At the end of the treatment period, the animals will be submitted to the MRI to analyze the integrity of white matter and gray matter volume and gray matter of the brain areas of the pain matrix as thalamus, somatosensory cortex, insular cortex, cingulate cortex, prefrontal cortex -motor, primary motor cortex, supplementary motor area, prefrontal cortex, posterior parietal cortex, amygdala and midbrain. Thereafter the animals will be subjected to testing immunohistochemistry for Fos protein in the same areas and for immunoblotting NGF and substance P in the spinal cord and posterior root ganglia. The obtained data will further elucidate the understanding of neuronal plasticity involved in chronic pain processes and increase knowledge of the role of NGF for further development and refinement of the treatment of persistent pain. (AU) | |
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