Advanced search
Start date
Betweenand

Magnetic resonance and supraspinal neuronal activation in chronic neuropathic pain model in rats

Grant number: 12/24408-6
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): April 01, 2013
Effective date (End): November 30, 2016
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal researcher:Marucia Chacur
Grantee:Joyce Teixeira da Silva
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):14/20983-1 - Analysis of brain changes by magnetic resonance imaging after chronic pain in rodents, BE.EP.DD

Abstract

The analysis of magnetic resonance imaging called "pain matrix", i.e. brain areas related to pain have attracted great interest from the scientific community, since the range of animal models of pain facilitation and predictive data to provide a therapeutic agents vast knowledge central nervous system. The nerve growth factor (NGF) is a neurotrophin that is closely linked to the induction and maintenance of painful processes. Being the main factor related to increased substance P, an important mediator of nociception. Anti-NGF treatment is a search for various painful pathologies, however, its participation in chronic neuropathic pain with the analysis of magnetic resonance imaging and immunogold labeling for Fos is unknown. In order to elucidate the mechanisms involved in both the model of peripheral neuropathy and post-treatment with anti-NGF will be used adult male Wistar rats subjected to induction of neuropathic pain by sciatic nerve surgery. False-operated animals submitted the same incision that operated animals, but without nerve ligation and naive animals without any surgical intervention, will be used as control. After the fourteenth day, the animals are treated with anti-NGF or vehicle. To assess the nociceptive threshold will be determined mechanical hyperalgesia, thermal hyperalgesia and allodynia before any procedure after induction of neuropathy and during treatment. At the end of the treatment period, the animals will be submitted to the MRI to analyze the integrity of white matter and gray matter volume and gray matter of the brain areas of the pain matrix as thalamus, somatosensory cortex, insular cortex, cingulate cortex, prefrontal cortex -motor, primary motor cortex, supplementary motor area, prefrontal cortex, posterior parietal cortex, amygdala and midbrain. Thereafter the animals will be subjected to testing immunohistochemistry for Fos protein in the same areas and for immunoblotting NGF and substance P in the spinal cord and posterior root ganglia. The obtained data will further elucidate the understanding of neuronal plasticity involved in chronic pain processes and increase knowledge of the role of NGF for further development and refinement of the treatment of persistent pain. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, JOYCE T.; EVANGELISTA, BIANCA G.; VENEGA, RAFAEL A. G.; SEMINOWICZ, DAVID A.; CHACUR, MARUCIA. Anti-NGF treatment can reduce chronic neuropathic pain by changing peripheral mediators and brain activity in rats. Behavioural Pharmacology, v. 30, n. 1, p. 79-88, FEB 2019. Web of Science Citations: 0.
DA SILVA, J. T.; EVANGELISTA, B. G.; VENEGA, R. A. G.; OLIVEIRA, M. E.; CHACUR, M. EARLY AND LATE BEHAVIORAL CHANGES IN SCIATIC NERVE INJURY MAY BE MODULATED BY NERVE GROWTH FACTOR AND SUBSTANCE P IN RATS: A CHRONIC CONSTRICTION INJURY LONG-TERM EVALUATION. JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS, v. 31, n. 2, p. 309-319, APR-JUN 2017. Web of Science Citations: 4.
GIARDINI, ALINE CAROLINA; DOS SANTOS, FABIO MARTINEZ; DA SILVA, JOYCE TEIXEIRA; DE OLIVEIRA, MARA EVANY; MARTINS, DANIEL OLIVEIRA; CHACUR, MARUCIA. Neural Mobilization Treatment Decreases Glial Cells and Brain-Derived Neurotrophic Factor Expression in the Central Nervous System in Rats with Neuropathic Pain Induced by CCI in Rats. PAIN RESEARCH & MANAGEMENT, 2017. Web of Science Citations: 3.
SANTOS, FABIO MARTINEZ; GRECCO, LEANDRO HENRIQUE; PEREIRA, MARCELO GOMES; OLIVEIRA, MARA EVANY; ROCHA, PRISCILA ABREU; SILVA, JOYCE TEIXEIRA; MARTINS, DANIEL OLIVEIRA; MIYABARA, ELEN HARUKA; CHACUR, MARUCIA. The neural mobilization technique modulates the expression of endogenous opioids in the periaqueductal gray and improves muscle strength and mobility in rats with neuropathic pain. Behavioral and Brain Functions, v. 10, MAY 13 2014. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Joyce Teixeira da. Magnetic resonance imaging and supraspinal neuronal activation in chronic neuropathic pain model in rats.. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.