Cytokine profile in dengue virus infection: identification of potential biomarkers for diagnosis and prognosis

• Grant number: 12/24574-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2013
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Victor Hugo Aquino Quintana
• Grantee: Muhammad Nawaz
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Dengue is an infectious non-contagious disease caused by dengue viruses (DENV). About 50 to 100 million people are infected annually, making dengue a major public health concern especially in tropical and sub-tropical regions. Dengue has been recognized as a clinical entity since 1779, but up to now; the pathogenesis of infection is not completely understood. Currently, there are not available early laboratory markers that may indicate the risk of developing severe dengue. In the early hours of viral infection, the cytokines produced by cells that were infected or in contact with viral products, are vital in the conduct of the innate immune response. The infection of target cells such as dendritic cells, macrophages/monocytes, endothelial cells, neutrophils, fibroblasts, mast cells and hepatocytes in DENV infection, promotes a phenomenon known as "cytokine storm", highlighting the importance of these molecules in the pathogenesis of the disease. The aim of this study is to identify biomarkers of dengue with diagnostic and prognostic values, by determining the profile of cytokines in a panel of serum samples from dengue infected patients. The profile of cytokine will be determined by the cytokine microarray technology as well as by multi-analyte profiling beads (xMAP). (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMIREZ, MARCEL I.; AMORIM, MARIA G.; GADELHA, CATARINA; MILIC, IVANA; WELSH, JOSHUA A.; FREITAS, VANESSA M.; NAWAZ, MUHAMMAD; AKBAR, NAVEED; COUCH, YVONNE; MAKIN, LAURA; et al. Technical challenges of working with extracellular vesicles. NANOSCALE, v. 10, n. 3, p. 881-906, JAN 21 2018. (14/26897-0, 12/24574-3, 14/06863-3, 15/50257-3)

NAWAZ, MUHAMMAD; CAMUSSI, GIOVANNI; VALADI, HADI; NAZARENKO, IRINA; EKSTROM, KARIN; WANG, XIAOQIN; PRINCIPE, SIMONA; SHAH, NEELAM; ASHRAF, NAEEM M.; FATIMA, FARAH; et al. The emerging role of extracellular vesicles as biomarkers for urogenital cancers. NATURE REVIEWS UROLOGY, v. 11, n. 12, p. 688-701, DEC 2014. (12/24574-3)

FATIMA, FARAH; EKSTROM, KARIN; NAZARENKO, IRINA; MAUGERI, MARCO; VALADI, HADI; HILL, ANDREW F.; CAMUSSI, GIOVANNI; NAWAZ, MUHAMMAD. Non-coding RNAs in Mesenchymal Stem Cell-Derived Extracellular Vesicles: Deciphering Regulatory Roles in Stem Cell Potency, Inflammatory Resolve, and Tissue Regeneration. FRONTIERS IN GENETICS, v. 8, OCT 25 2017. (12/24574-3)

NAWAZ, MUHAMMAD; SHAH, NEELAM; ZANETTI, BRUNA RIEDO; MAUGERI, MARCO; SILVESTRE, RENATA NACASAKI; FATIMA, FARAH; NEDER, LUCIANO; VALADI, HADI. Extracellular Vesicles and Matrix Remodeling Enzymes: The Emerging Roles in Extracellular Matrix Remodeling, Progression of Diseases and Tissue Repair. CELLS, v. 7, n. 10, OCT 2018. (12/24574-3)

MATEESCU, BOGDAN; KOWAL, EMMA J. K.; VAN BALKOM, BAS W. M.; BARTEL, SABINE; BHATTACHARYYA, SUVENDRA N.; BUZAS, EDIT I.; BUCK, AMY H.; DE CANDIA, PAOLA; CHOW, FRANKLIN W. N.; DAS, SAUMYA; et al. Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper. JOURNAL OF EXTRACELLULAR VESICLES, v. 6, 2017. (12/24574-3)

STATELLO, LUISA; MAUGERI, MARCO; GARRE, ELENA; NAWAZ, MUHAMMAD; WAHLGREN, JESSICA; PAPADIMITRIOU, ALEXANDROS; LUNDQVIST, CHRISTINA; LINDFORS, LENNART; COLLEN, ANNA; SUNNERHAGEN, PER; et al. Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes. PLoS One, v. 13, n. 4, APR 24 2018. (12/24574-3)

NAWAZ, MUHAMMAD; FATIMA, FARAH. Extracellular Vesicles, Tunneling Nanotubes, and Cellular Interplay: Synergies and Missing Links. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 4, 2017. (12/24574-3)

FATIMA, FARAH; NAWAZ, MUHAMMAD. Long Distance Metabolic Regulation through Adipose-Derived Circulating Exosomal miRNAs: A Trail for RNA-Based Therapies?. FRONTIERS IN PHYSIOLOGY, v. 8, AUG 2 2017. (12/24574-3)

QIAN, CHAO-NAN (MILES); PEZZELLA, FRANCESCO; ALBERTI, SAVERIO; KRISHNATREYA, MANIGREEVA; NAWAZ, MUHAMMAD; WANG, MEI; CHEN, SHUAI. The 150 most important questions in cancer research and clinical oncology series: questions 15-24 Edited by Chinese Journal of Cancer. CHINESE JOURNAL OF CANCER, v. 36, p. 7-pg., 2017-04-05. (12/24574-3)