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Evaluation of different cryopreservation methods and the effect of treatment with anti-oxidant agents in the post-thaw seminal characteristics of howler monkeys (Alouatta caraya)

Grant number: 12/24709-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2013
Effective date (End): June 30, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Mario Binelli
Grantee:Fernanda Maria de Carvalho
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):14/15847-1 - Cryopreservation of spermatozoa from Rhesus macaques (Macaca mulatta) and assessment of frozen-thawed semen quality by IVF, BE.EP.DR


The constant threats to non-human primate survival makes it necessary to intervene with Assisted Reproductive Technologies. A recent study assessed different cryopreservation protocols for Alouatta caraya semen. Although none of the results were satisfying, the best results were obtained using an egg yolk-based extender with 4% glycerol. The present project was elaborated based on the results of the latter and on other studies using different species. The study will be divided in two experiments: Experiment I - determination of glycerol concentration and freezing rate for Alouatta caraya semen; Experiment II - evaluation of the effects of adding DHA and vitamin E to Alouatta caraya semen. A total of seven males will be used, two from Fundação Parque Zoológico de São Paulo, São Paulo - SP and five from Centro Nacional de Primatas, Ananindeua - PA. In order to compare the different protocols, the following parameter will be assessed: sperm motility, sperm morphology, plasma membrane integrity, acrosome integrity, mitochondrial activity, susceptibility to oxidative stress (TBARS), and susceptibility of the chromatin to acid denaturation (SCSA). Additionally, Fourier Transform Infrared Spectroscopy (FTIR) will be used to obtain data regarding sperm composition before and after cryopreservation. Data will be analysed using SAS system for Windows.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CARVALHO, Fernanda Maria de. Cryopreservation of non-human primate sperm. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia São Paulo.

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