Design and transfection of microRNAs as a therapeutical strategy in vulvar carcinomas

• Grant number: 13/04075-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 03, 2013
• End date: December 02, 2013
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Rafael Malagoli Rocha
• Grantee: Beatriz de Melo Maia
• Supervisor: George A. Calin
• Host Institution: A C Camargo Cancer Center. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil
• Institution abroad: University of Texas MD Anderson Cancer Center (MD Anderson), United States
• Associated to the scholarship: 11/18065-6 - Evaluation of the role of microRNA in vulvar carcinoma, BP.DR

Abstract

Recent findings obtained in ongoing PhD project have demonstrated that miR-133a has clinical importance in vulva carcinomas, a rare type of gynecological tumor in which lack of biological information becomes strong the need to identify molecules that facilitate the understanding of operating mechanisms in this tumor type, such as microRNAs, which deregulated expression has been demonstrated as one of the major determinants of tumorigenesis. Objectives: This project aims to elucidate the role of miR-133a and the search for accurate therapeutic strategies based on the inhibition of this microRNA, in order to provide a new biomarker with prognostic, predictive and therapeutic potential in vulvar carcinomas. Methods: Vectors containing sponge regions to miR-133a and control sequences downstream of the GFP reporter gene (green fluorescent protein) will be designed, synthesized, and transfected into vulvar carcinoma cell line SW-954. The investigation of miR-133a activity in the established cell line with vectors will be performed by luciferase assays, whereby the efficiency will be assessed from inhibition of microRNA of interest. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAIA, BEATRIZ M.; ROCHA, RAFAEL M.; CALIN, GEORGE A.. Clinical significance of the interaction between non-coding RNAs and the epigenetics machinery Challenges and opportunities in oncology. Epigenetics, v. 9, n. 1, SI, p. 75-80, JAN 1 2014. (13/04075-5)

MAIA, BEATRIZ DE MELO; LING, HUI; MONROIG, PALOMA; CICCONE, MARIA; SOARES, FERNANDO A.; CALIN, GEORGE A.; ROCHA, RAFAEL M.. Design of a miRNA sponge for the miR-17 miRNA family as a therapeutic strategy against vulvar carcinoma. MOLECULAR AND CELLULAR PROBES, v. 29, n. 6, p. 420-426, DEC 2015. (13/04075-5, 11/18065-6)

MAIA, BEATRIZ DE MELO; FONTES, ADRIANA MAZEGA; LAVORATO-ROCHA, ANDRE MOURAO; RODRIGUES, IARA SANT'ANA; DE BROT, LOUISE; BAIOCCHI, GLAUCO; STIEPCICH, MONICA MARIA; SOARES, FERNANDO AUGUSTO; ROCHA, RAFAEL MALAGOLI. EGFR expression in vulvar cancer: clinical implications and tumor heterogeneity. HUMAN PATHOLOGY, v. 45, n. 5, p. 917-925, MAY 2014. (13/04075-5)

MAIA, BEATRIZ DE MELO; RODRIGUES, IARA SANTANA; AKAGI, ERICA MIE; DO AMARAL, NAYRA SOARES; LING, HUI; MONROIG, PALOMA; SOARES, FERNANDO AUGUSTO; CALIN, GEORGE ADRIAN; ROCHA, RAFAEL MALAGOLI. MiR-223-5p works as an oncomiR in vulvar carcinoma by TP63 suppression. ONCOTARGET, v. 7, n. 31, p. 49217-49231, AUG 2 2016. (13/04075-5, 11/18065-6)

MAIA, BEATRIZ M.; ROCHA, RAFAEL M.; CALIN, GEORGE A.. Clinical significance of the interaction between non-coding RNAs and the epigenetics machinery Challenges and opportunities in oncology. Epigenetics, v. 9, n. 1, p. 6-pg., 2014-01-01. (13/04075-5)