Comparative analysis of rabies virus glycoprotein expression in two culture media

• Grant number: 13/01334-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2013
• End date: March 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Renato Mancini Astray
• Grantee: Aline Heimy Morimoto
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado)

Abstract

The objective of this research is to evaluate the production of recombinant rabies virus glycoprotein by S2 Drosophila melanogaster cells, cultivated in two different culture media. Firstly the cells will be adapted to growth in each medium and stored as a work bank. The RVGP will be produced in kinetic assays, when the consumption of glucose, glutamine and the production of lactate will be evaluated as well. The RVGP production will be characterized in terms of volumetric and specific productivity, and in the context of the consumption of substrates and production of metabolities. The RVGP produced in these assays will be purified with the add of its histidine tag and analysed by SDS-PAGE and Western-blotting. (AU)