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Comparative analysis of rabies virus glycoprotein expression in two culture media

Grant number: 13/01334-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2013
Effective date (End): March 31, 2014
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Renato Mancini Astray
Grantee:Aline Heimy Morimoto
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The objective of this research is to evaluate the production of recombinant rabies virus glycoprotein by S2 Drosophila melanogaster cells, cultivated in two different culture media. Firstly the cells will be adapted to growth in each medium and stored as a work bank. The RVGP will be produced in kinetic assays, when the consumption of glucose, glutamine and the production of lactate will be evaluated as well. The RVGP production will be characterized in terms of volumetric and specific productivity, and in the context of the consumption of substrates and production of metabolities. The RVGP produced in these assays will be purified with the add of its histidine tag and analysed by SDS-PAGE and Western-blotting.