Production of recombinant proteins in BHK-21 cells cultured in serum-free medium
| Grant number: | 13/01334-0 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | April 01, 2013 |
| Effective date (End): | March 31, 2014 |
| Field of knowledge: | Biological Sciences - Microbiology |
| Principal Investigator: | Renato Mancini Astray |
| Grantee: | Aline Heimy Morimoto |
| Home Institution: | Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil |
Abstract The objective of this research is to evaluate the production of recombinant rabies virus glycoprotein by S2 Drosophila melanogaster cells, cultivated in two different culture media. Firstly the cells will be adapted to growth in each medium and stored as a work bank. The RVGP will be produced in kinetic assays, when the consumption of glucose, glutamine and the production of lactate will be evaluated as well. The RVGP production will be characterized in terms of volumetric and specific productivity, and in the context of the consumption of substrates and production of metabolities. The RVGP produced in these assays will be purified with the add of its histidine tag and analysed by SDS-PAGE and Western-blotting. | |