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Role of apolipoprotein E (apoE) isoforms in cholesterol flux of Leishmania spp. infected macrophages

Grant number: 12/24452-5
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): February 15, 2014
Effective date (End): February 14, 2015
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Paulo Eduardo Martins Ribolla
Grantee:Diego Peres Alonso
Supervisor abroad: Toni Aebischer
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Local de pesquisa : Robert Koch Institute (RKI), Germany  
Associated to the scholarship:10/19973-0 - The role of peroxisome proliferator-activated receptors alpha (PPARalfa) as a genetic risk factor in visceral leishmaniasis, BP.PD

Abstract

Lipid disorders have been reported in human patients and even in domestic dogs with active visceral leishmaniasis (VL). The LV in Brazil is a parasitic disease caused by infection of host macrophages by the protozoan Leishmania infantum chagasi and transmitted by the sandfly Lutzomyia longipalpis, the main reservoirs are wild canids and domestic dogs. Several studies have shown a proven relationship between serum lipid levels and infection by Leishmania infantum chagasi, and others more recently shown that specific genotypes of apoE are associated with susceptibility to LV, so the aim of this project is to evaluate the role of different isoforms of apoE on the viability of Leishmania spp. within the host cell taking into account genotype-specific effects of this protein in the flow of cholesterol in macrophages. Furthermore, the possible differences in flow cholesterol are correlated with alterations in membrane integrity of the infected macrophage, since cholesterol plays an essential role in the organization of "lipid rafts" fundamental structures in the process of signal transduction and antigens presentation. (AU)