Alteration of rat primordial germ cell epigenetic reprogramming and its influence on the expression of the pluripotent marker SSEA1.

• Grant number: 12/25087-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2013
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Morphology - Embryology
• Principal Investigator: Taiza Stumpp Teixeira
• Grantee: Isabelle Hernandez Cantão
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Germ cells (GC) are the precursors of the gametes. During their embryonic development, the GC undergo a wide epigenetic reprogramming, when DNA methylation is mostly erased. At this moment, the GC temporarily present pluripotency characteristics and can be transformed into stem cells in vitro. This capacity have been studied in mice, but not in rats. Our group has been showing that rat germ cell development is different from mouse and show some similarities with humans, what make the rat a good model to study germ cell development. One difference between mice and rats is the difficulties to get stem cells from the rat PGC, what has been well described in mice. Studies form our group have been suggesting that this can be due to the differences in the timing of the epigenetic reprogramming in these two species. Thus, the aim of this study is to change the period of epigenetic reprogramming in the rat PGC to extend the period when these cells show pluripotency characteristics. Pregnant Wistar rats will receive 5-Aza-deoxicitidina (5-Aza, 2mg/Kg) at 12dpc. The epigenetic reprogramming and Ssea1 expression will be investigated. This study can provide data to improve the derivation of pluripotent stem cells from rat PGC. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANTAO, ISABELLE HERNANDEZ; TESSER, RENATO BORGES; STUMPP, TAIZA. An Initial Investigation of an Alternative Model to Study rat Primordial Germ Cell Epigenetic Reprogramming. BIOLOGICAL PROCEDURES ONLINE, v. 19, AUG 3 2017. (11/07078-0, 12/25087-9, 12/01024-8)