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Imprinted genes in the process of pluripontency acquisition on bovine cells

Grant number: 12/24766-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2013
Effective date (End): October 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Felipe Perecin
Grantee:Marina Amaro de Lima
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The discovery of the mechanisms of pluripotency induction lead to great changes on the studies of the cellular events related to the acquisition of pluripotency and nuclear reprogramming. However, although the generation of iPS cells has already been reported in several species, the outcomes in bovines are still unpredictable. Recently, some researchers have correlated the reprogramming success with the adequate reprogramming of some imprinted genes. Thus, the present work aim to study the effects of different protocols of pluripotency induction on the epigenetic status and expression of some imprinted genes in cattle. Using a model based on single nucleotide polymorphism between alleles of IGF2R and H19 genes, that have known importance on embryo and fetal development, its patterns of expression on bovine fibroblasts will be evaluated throughout the generation of pluripotent cells using different protocols. The results of this study will increase the knowledge on the mechanism driving the induction of pluripontency in the bovine specie, as well as the role of the imprinted genes on this process.