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Involvement of cannabinoid system in the oxidative and inflammatory processes related to neurodegeneration in vitro

Grant number: 13/02916-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2013
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Andréa da Silva Torrão
Grantee:Hadassa Batinga da Silva
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The cannabinoid system has been implicated as an important neuromodulation system in several experimental models of neuronal injury both acute and chronic type, which many studies suggest a neuroprotective action. Parkinson's disease (PD), characterized by loss of dopaminergic neurons in the substantia nigra, yet its etiology is unknown. Nevertheless, several factors seem to be involved as mitochondrial dysfunction, oxidative stress, and neuroinflammation. Studies on the relations of the cannabinoid system and DP, however, are still very controversial, partly because the literature related to possible neuroprotective role of the cannabinoid system are still incipient. The overall objectives of this project are to investigate the involvement of cannabinoid system in an in vitro model of neurodegeneration, which evaluate the parameters of oxidative stress and neuroinflammation as a way to understand the contribution of each to the progression of neurodegenerative diseases generally and in particular PD and the role of the cannabinoid system. For this, we use a strain of neuroblastoma cells to be differentiated into dopaminergic and treated with different inducers of neuronal damage such as 6-OHDA, H2O2 and LPS conjugated to treatment with agonist and antagonist cannabinoids. Parameters will be assessed cell viability marker proteins in addition to oxidative stress, neuronal death and survival, by enzymatic methods, Western blot, immuno-cytochemical and PCR. (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Hadassa Batinga da. Participation of the cannabinoid system in oxidative and inflammatory processes related to neurodegeneration in vitro.. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas São Paulo.

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