Full text
| |
| Author(s): |
Hadassa Batinga da Silva
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-12-08 |
| Examining board members: |
Andréa da Silva Torrão;
José Donato Júnior;
Manoel de Arcisio Miranda Filho;
Tânia Araújo Viel
|
| Advisor: | Andréa da Silva Torrão |
| Abstract | |
The CB1 receptor activation leads to modulation of intracellular processes that change the cellular response according to the stimulus, as well as being involved in mechanisms of proliferation, differentiation, cell movement and death. The present study evaluated the participation of this system in oxidative and inflammatory processes related to neurodegeneration in vitro. We have used the Neuro2A neuroblastoma lineage, which those were differentiated into dopaminergic cells, and exposed to 6OHDA, H2O2 and LPS. They were co-treated with ACEA, CB1 receptor agonist, and AM251, the CB1 receptor antagonist/inverse agonist, for 24 hours. We used functional parameters of cell viability, production of reactive oxygen species and protein analyses by western blot. Treatment with ACEA or ACEA/AM251 produced an increase in cell viability; reduced production of reactive oxygen species and activation of the ERK1/2 protein, in addition to inhibition of cell death by decreasing the expression of caspase 3 in all three models proposed. We concluded that chosen cannabinoids were able to protect dopaminergic cells from oxidative damage and inflammation through the increased cell survival by decreasing the production of ROS. (AU) | |
| FAPESP's process: | 13/02916-2 - Involvement of cannabinoid system in the oxidative and inflammatory processes related to neurodegeneration in vitro |
| Grantee: | Hadassa Batinga da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |